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Protein

Protein RUFY3

Gene

Rufy3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the generation of neuronal polarity formation and axon growth (PubMed:17439943). Implicated in the formation of a single axon by developing neurons (PubMed:17439943). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (PubMed:17439943). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (By similarity). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (By similarity).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RUFY3Curated
Alternative name(s):
RUN and FYVE domain-containing protein 3Imported
Rap2-interacting protein xBy similarity
Short name:
RIPxBy similarity
Single axon-regulated protein1 Publication
Short name:
Singar1 Publication
Gene namesi
Name:Rufy3Imported
Synonyms:RipxBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1565242. Rufy3.

Subcellular locationi

  • Cytoplasm By similarity
  • Endomembrane system By similarity
  • Cell projectioninvadopodium By similarity
  • Perikaryon 1 Publication
  • Cell projection 1 Publication
  • Cell projectiongrowth cone By similarity
  • Cell projectionfilopodium By similarity
  • Cell projectionlamellipodium By similarity

  • Note: Localized in F-actin-enriched filopodia and lamellipodia at axonal growth cones (PubMed:17439943). Colocalizes with PAK1, F-actin, myosins and integrins in invadopodia at the cell periphery (By similarity). Colocalized with Ras-related Rab-5 proteins in cytoplasmic vesicles (By similarity). Accumulates in axon growth cones in a F-actin-dependent manner (By similarity). Colocalized with FSCN1 and F-actin at filipodia and lamellipodia of axonal growth cones (By similarity). Colocalized with DBN1 and F-actin at transitional domain of the axonal growth cone (By similarity).By similarity1 Publication

GO - Cellular componenti

  • axon Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • dendrite Source: UniProtKB
  • endomembrane system Source: UniProtKB-SubCell
  • filopodium Source: HGNC
  • growth cone Source: HGNC
  • invadopodium Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • membrane Source: UniProtKB-KW
  • perikaryon Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Protein RUFY3PRO_0000245836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphothreonineCombined sources
Modified residuei12 – 121PhosphothreonineCombined sources
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei51 – 511PhosphothreonineCombined sources

Post-translational modificationi

Isoform 1 is partially phosphorylated (PubMed:17439943). Phosphorylated by PAK1 (By similarity).By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5FVJ0.
PRIDEiQ5FVJ0.

PTM databases

iPTMnetiQ5FVJ0.

Expressioni

Tissue specificityi

Expressed in brain (at protein level) (PubMed:17439943).1 Publication

Developmental stagei

Low level expression in brain up to E15, higher level expression from E18 to P14 with highest level around P4 (PubMed:17439943). Low level expression in adult brain (PubMed:17439943). Low level expression in hippocampal neurons up to stage 2 in culture (PubMed:17439943). Higher level from stage 3 with highest level of isoform 1 on day 7 in vitro (DIV7) (PubMed:17439943). Phosphorylated isoform 1 from DIV7 with high level up to DIV28, isoform 2 detectable from stage 3 to DIV7 (PubMed:17439943).1 Publication

Inductioni

Up-regulated during neuronal polarization (PubMed:17439943).1 Publication

Gene expression databases

ExpressionAtlasiQ5FVJ0. baseline and differential.
GenevisibleiQ5FVJ0. RN.

Interactioni

Subunit structurei

Interacts with PIK3CA and PIK3R1 (PubMed:17439943). Interacts with PAK1 (By similarity). Interacts (via C-terminus) with Ras-related Rab-5 proteins (By similarity). Interacts (via C-terminus) with Ras-related Rap-2 proteins (By similarity). Interacts (via N-terminus) with FSCN1; this interaction induces neuron axon development (By similarity). Interacts with DBN1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi262306. 2 interactions.
STRINGi10116.ENSRNOP00000020608.

Structurei

3D structure databases

ProteinModelPortaliQ5FVJ0.
SMRiQ5FVJ0. Positions 65-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 227133RUNPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili267 – 464198Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 RUN domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000030913.
HOVERGENiHBG057053.
InParanoidiQ5FVJ0.
OMAiIKLERVC.
OrthoDBiEOG722J98.

Family and domain databases

InterProiIPR004012. Run_dom.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5FVJ0-1) [UniParc]FASTAAdd to basket

Also known as: Singar11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSALTPPTDM PTPTTDKITQ AAMETIYLCK FRVSMDGEWL CLRELDDISL
60 70 80 90 100
TPDPEPTHED PNYLMANERM NLMNMAKLSI KGLIESALNL GRTLDSDYAP
110 120 130 140 150
LQQFFVVMEH CLKHGLKAKK TFLGQNKSFW GPLELVEKLV PEAAEITASV
160 170 180 190 200
KDLPGLKTPV GRGRAWLRLA LMQKKLSEYM KALINKKELL SEFYEANALM
210 220 230 240 250
MEEEGAIIAG LLVGLNVIDA NFCMKGEDLD SQVGVIDFSM YLKDGNSSKG
260 270 280 290 300
SEGDGQITAI LDQKNYVEEL NRHLNATVNN LQAKVDALEK SNTKLTEELA
310 320 330 340 350
VANNRIITLQ EEMERVKEES SYLLESNRKG PKQDRTAEGQ ALSEARKHLK
360 370 380 390 400
EETQLRLDVE KELELQISMR QEMELAMKML EKDVCEKQDA LVSLRQQLDD
410 420 430 440 450
LRALKHELAF KLQSSDLGVK QKSELNSRLE EKTNQMAATI KQLEQSEKDL
460
VKQAKTLNSA ANKLIPKHH
Length:469
Mass (Da):52,907
Last modified:March 1, 2005 - v1
Checksum:i98580BE52F52BC04
GO
Isoform 2 (identifier: Q5FVJ0-2) [UniParc]FASTAAdd to basket

Also known as: Singar21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     60-60: D → DSWEDLTDLVEQVRADPED

Show »
Length:487
Mass (Da):55,006
Checksum:i57FC104059BA9CF0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei60 – 601D → DSWEDLTDLVEQVRADPED in isoform 2. 1 PublicationVSP_026950

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF538802 mRNA. Translation: ABP99060.1.
EF577045 mRNA. Translation: ABQ45403.1.
BC089952 mRNA. Translation: AAH89952.1.
RefSeqiNP_001020298.1. NM_001025127.1. [Q5FVJ0-1]
XP_008768270.1. XM_008770048.1. [Q5FVJ0-2]
UniGeneiRn.228806.

Genome annotation databases

EnsembliENSRNOT00000004776; ENSRNOP00000004776; ENSRNOG00000003428. [Q5FVJ0-1]
ENSRNOT00000020608; ENSRNOP00000020608; ENSRNOG00000003428. [Q5FVJ0-2]
GeneIDi360921.
KEGGirno:360921.
UCSCiRGD:1565242. rat. [Q5FVJ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF538802 mRNA. Translation: ABP99060.1.
EF577045 mRNA. Translation: ABQ45403.1.
BC089952 mRNA. Translation: AAH89952.1.
RefSeqiNP_001020298.1. NM_001025127.1. [Q5FVJ0-1]
XP_008768270.1. XM_008770048.1. [Q5FVJ0-2]
UniGeneiRn.228806.

3D structure databases

ProteinModelPortaliQ5FVJ0.
SMRiQ5FVJ0. Positions 65-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262306. 2 interactions.
STRINGi10116.ENSRNOP00000020608.

PTM databases

iPTMnetiQ5FVJ0.

Proteomic databases

PaxDbiQ5FVJ0.
PRIDEiQ5FVJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004776; ENSRNOP00000004776; ENSRNOG00000003428. [Q5FVJ0-1]
ENSRNOT00000020608; ENSRNOP00000020608; ENSRNOG00000003428. [Q5FVJ0-2]
GeneIDi360921.
KEGGirno:360921.
UCSCiRGD:1565242. rat. [Q5FVJ0-1]

Organism-specific databases

CTDi22902.
RGDi1565242. Rufy3.

Phylogenomic databases

eggNOGiKOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000030913.
HOVERGENiHBG057053.
InParanoidiQ5FVJ0.
OMAiIKLERVC.
OrthoDBiEOG722J98.

Miscellaneous databases

NextBioi674606.
PROiQ5FVJ0.

Gene expression databases

ExpressionAtlasiQ5FVJ0. baseline and differential.
GenevisibleiQ5FVJ0. RN.

Family and domain databases

InterProiIPR004012. Run_dom.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
[Graphical view]
PROSITEiPS50826. RUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Singar1, a novel RUN domain-containing protein, suppresses formation of surplus axons for neuronal polarity."
    Mori T., Wada T., Suzuki T., Kubota Y., Inagaki N.
    J. Biol. Chem. 282:19884-19893(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 82-92; 139-151; 265-284; 306-328; 348-356 AND 403-411, FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH PIK3CA AND PIK3R1, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5; THR-12; SER-49 AND THR-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRUFY3_RAT
AccessioniPrimary (citable) accession number: Q5FVJ0
Secondary accession number(s): A5HLX7, A5JUR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2005
Last modified: May 11, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Hippocampal neurons with reduced levels of RUFY3 show an increase in formation of additional axons (PubMed:17439943). This effect is suppressed in the presence of the PI3K inhibitor LY294002 and enhanced by overexpression of SHTN1 (PubMed:17439943). Overexpression of RUFY3 suppresses additional axons formed upon overexpression of SHTN1 (PubMed:17439943).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.