Q5FVH2 (PLD3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D3
Short name=PLD 3
EC=3.1.4.4

Alternative name(s):
Choline phosphatase 3
Phosphatidylcholine-hydrolyzing phospholipase D3

Gene names

Name:

Pld3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Endoplasmic reticulum Membrane
Domain	Repeat Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Phospholipase D3		PRO_0000280330
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	60 – 488	429	Lumenal Potential
	Domain	194 – 221	28	PLD phosphodiesterase 1
	Domain	409 – 435	27	PLD phosphodiesterase 2
Sites
	Active site	199	1	Potential
	Active site	201	1	Potential
	Active site	206	1	Potential
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5FVH2 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 081AA487F575747A	FASTA	488	54,399
	10 20 30 40 50 60 MKPKLMYQEL KVPVEEPAGE LPMNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW 70 80 90 100 110 120 EYGDLHLFGP NQHPAPCYDP CEAVLVESIP EGLEFPNATT SNPSTSQAWL GLLAGAHSSL 130 140 150 160 170 180 DIASFYWTLT NNDTHTQEPS AQQGEEVLQQ LQALAPRGVK VRIAVSKPNG PLADLQSLLQ 190 200 210 220 230 240 SGAQVRMVDM QKLTHGVLHT KFWVVDQTHF YLGSANMDWR SLTQVKELGV VMYNCSCLAR 250 260 270 280 290 300 DLTKIFEAYW FLGQAGSSIP STWPRPFDTR YNQETPMEIC LNGTPALAYL ASAPPPLCPG 310 320 330 340 350 360 GRTPDLKALL SVVDNARSFI YIAVMNYLPT MEFSHPRRFW PAIDDGLRRA AYERGVKVRL 370 380 390 400 410 420 LISCWGHSEP SMRSFLLSLA ALRDNHTHSD IQVKLFVVPA DEAQARIPYA RVNHNKYMVT 430 440 450 460 470 480 ERTTYIGTSN WSGSYFTETA GTSLLVTQNG HGGLRSQLEA VFLRDWESPY SHNLDTSADS VGNACRLL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC089987 mRNA. Translation: AAH89987.1.
IPI	IPI00365672.
RefSeq	NP_001012167.1. NM_001012167.1.
UniGene	Rn.128036.
3D structure databases
ProteinModelPortal	Q5FVH2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5FVH2. 1 interaction.
PTM databases
PhosphoSite	Q5FVH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000057177; ENSRNOP00000054004; ENSRNOG00000018390.
GeneID	361527.
KEGG	rno:361527.
NMPDR	fig|10116.3.peg.1366.
UCSC	NM_001012167. rat.
Organism-specific databases
CTD	23646.
RGD	1308248. Pld3.
Phylogenomic databases
eggNOG	roNOG08898.
GeneTree	ENSGT00390000009798.
HOVERGEN	HBG052880.
InParanoid	Q5FVH2.
OMA	IDIASFY.
OrthoDB	EOG4M65HG.
PhylomeDB	Q5FVH2.
Gene expression databases
ArrayExpress	Q5FVH2.
Genevestigator	Q5FVH2.
Family and domain databases
InterPro	IPR001736. PLipase_D/transphosphatidylase. IPR013582. PLipase_D_envelope. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 1 hit. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	676617.

Entry information

Entry name

PLD3_RAT

Accession

Primary (citable) accession number: Q5FVH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2005
Last modified:	October 19, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program