Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5FUW9

- DAPA_GLUOX

UniProt

Q5FUW9 - DAPA_GLUOX

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (10 Jun 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei49 – 491Part of a proton relay during catalysisUniRule annotation
    Binding sitei50 – 501PyruvateUniRule annotation
    Sitei112 – 1121Part of a proton relay during catalysisUniRule annotation
    Active sitei138 – 1381Proton donor/acceptorUniRule annotation
    Active sitei166 – 1661Schiff-base intermediate with substrateUniRule annotation
    Binding sitei208 – 2081Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciGOXY290633:GHB3-61-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:GOX0061
    OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
    Taxonomic identifieri290633 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
    ProteomesiUP000006375: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2972974-hydroxy-tetrahydrodipicolinate synthasePRO_0000340955Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi290633.GOX0061.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FUW9.
    SMRiQ5FUW9. Positions 6-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    KOiK01714.
    OMAiMLYSGDD.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00665. DHDPS_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5FUW9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADTSMYQGS LTALVTPMHT DGRIDFDVSA KLIERQIEAG TTALIPAGTT    50
    GESPTLSHEE HGRVVAHCVE TANGRVRVMA GAGSNSTSEA VLMAKHAHSV 100
    GADSVLVVVP YYNKPTQEGL YRHFMTIADA TPLPMFLYCI PGRSVVDISV 150
    ATMARLAEHP NIVGTKDATA NMARPIAVRR AVDKPFNQLS GDDNSVLSFL 200
    AAGGDGCIGV TSNVVPHLCA DMHLAWQEGR IEDAIAIQDH LTPLHDAMFM 250
    ESNPGPVKYA LSRLGLCNAT LRLPLVEPQE ETRRAIDAAL RSLELLD 297
    Length:297
    Mass (Da):31,818
    Last modified:June 10, 2008 - v2
    Checksum:iE4EF224207A45146
    GO

    Sequence cautioni

    The sequence AAW59858.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000009 Genomic DNA. Translation: AAW59858.1. Different initiation.
    RefSeqiYP_190514.1. NC_006677.1.

    Genome annotation databases

    EnsemblBacteriaiAAW59858; AAW59858; GOX0061.
    GeneIDi3249160.
    KEGGigox:GOX0061.
    PATRICi32607784. VBIGluOxy81109_0286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000009 Genomic DNA. Translation: AAW59858.1 . Different initiation.
    RefSeqi YP_190514.1. NC_006677.1.

    3D structure databases

    ProteinModelPortali Q5FUW9.
    SMRi Q5FUW9. Positions 6-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290633.GOX0061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW59858 ; AAW59858 ; GOX0061 .
    GeneIDi 3249160.
    KEGGi gox:GOX0061.
    PATRICi 32607784. VBIGluOxy81109_0286.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    KOi K01714.
    OMAi MLYSGDD.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci GOXY290633:GHB3-61-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00665. DHDPS_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
      Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
      Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 621H.

    Entry informationi

    Entry nameiDAPA_GLUOX
    AccessioniPrimary (citable) accession number: Q5FUW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3