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Q5FUH9 (RNH_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease H

Short name=RNase H
EC=3.1.26.4
Gene names
Name:rnhA
Ordered Locus Names:GOX0177
OrganismGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]
Taxonomic identifier290633 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00042

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00042

Cofactor

Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00042

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00042.

Sequence similarities

Belongs to the RNase H family.

Contains 1 RNase H domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA-DNA hybrid ribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Ribonuclease H HAMAP-Rule MF_00042
PRO_0000332608

Regions

Domain7 – 148142RNase H

Sites

Metal binding161Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding541Magnesium 1 By similarity
Metal binding761Magnesium 1 By similarity
Metal binding1401Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FUH9 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 05DD704AFE043CEB

FASTA15016,878
        10         20         30         40         50         60 
MSEASGERPR VEIWTDGGCK PNPGPGGWGA LLVCRGQEKE LLGGDPETTN NRMELTAAAE 

        70         80         90        100        110        120 
ALEALKRPCI VTLHTDSEYL RNGITRWHTG WVRRKWRNAA GDPVANMDLW QRILEAAKPH 

       130        140        150 
EIDWLWVKGH SGDENNERVD QLATRGREEL 

« Hide

References

[1]"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 621H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000009 Genomic DNA. Translation: AAW59967.1.
RefSeqYP_190623.1. NC_006677.1.

3D structure databases

ProteinModelPortalQ5FUH9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290633.GOX0177.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW59967; AAW59967; GOX0177.
GeneID3248733.
KEGGgox:GOX0177.
PATRIC32608030. VBIGluOxy81109_0402.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0328.
HOGENOMHOG000040465.
KOK03469.
OMARKQVEIF.
OrthoDBEOG696BTR.

Enzyme and pathway databases

BioCycGOXY290633:GHB3-177-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00042. RNase_H.
InterProIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
PROSITEPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNH_GLUOX
AccessionPrimary (citable) accession number: Q5FUH9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families