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Protein

Ribonuclease H

Gene

rnhA

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Magnesium 1UniRule annotation
Metal bindingi16 – 161Magnesium 2UniRule annotation
Metal bindingi54 – 541Magnesium 1UniRule annotation
Metal bindingi76 – 761Magnesium 1UniRule annotation
Metal bindingi140 – 1401Magnesium 2UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. nucleic acid binding Source: InterPro
  3. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:GOX0177
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
ProteomesiUP000006375 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Ribonuclease HPRO_0000332608Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290633.GOX0177.

Structurei

3D structure databases

ProteinModelPortaliQ5FUH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 148142RNase HUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FUH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEASGERPR VEIWTDGGCK PNPGPGGWGA LLVCRGQEKE LLGGDPETTN
60 70 80 90 100
NRMELTAAAE ALEALKRPCI VTLHTDSEYL RNGITRWHTG WVRRKWRNAA
110 120 130 140 150
GDPVANMDLW QRILEAAKPH EIDWLWVKGH SGDENNERVD QLATRGREEL
Length:150
Mass (Da):16,878
Last modified:March 1, 2005 - v1
Checksum:i05DD704AFE043CEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW59967.1.
RefSeqiYP_190623.1. NC_006677.1.

Genome annotation databases

EnsemblBacteriaiAAW59967; AAW59967; GOX0177.
KEGGigox:GOX0177.
PATRICi32608030. VBIGluOxy81109_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW59967.1.
RefSeqiYP_190623.1. NC_006677.1.

3D structure databases

ProteinModelPortaliQ5FUH9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290633.GOX0177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW59967; AAW59967; GOX0177.
KEGGigox:GOX0177.
PATRICi32608030. VBIGluOxy81109_0402.

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-177-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
    Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
    Nat. Biotechnol. 23:195-200(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 621H.

Entry informationi

Entry nameiRNH_GLUOX
AccessioniPrimary (citable) accession number: Q5FUH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2005
Last modified: April 1, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.