Q5FUH9 (RNH_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease H
Short name=RNase H
EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	GOX0177

Organism

Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

290633 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter › Gluconobacter oxydans

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00042
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00042
Cofactor	Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00042.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Ribonuclease H HAMAP-Rule MF_00042		PRO_0000332608
Regions
	Domain	7 – 148	142	RNase H
Sites
	Metal binding	16	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	54	1	Magnesium 1 By similarity
	Metal binding	76	1	Magnesium 1 By similarity
	Metal binding	140	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FUH9 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 05DD704AFE043CEB	FASTA	150	16,878
	10 20 30 40 50 60 MSEASGERPR VEIWTDGGCK PNPGPGGWGA LLVCRGQEKE LLGGDPETTN NRMELTAAAE 70 80 90 100 110 120 ALEALKRPCI VTLHTDSEYL RNGITRWHTG WVRRKWRNAA GDPVANMDLW QRILEAAKPH 130 140 150 EIDWLWVKGH SGDENNERVD QLATRGREEL

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000009 Genomic DNA. Translation: AAW59967.1.
RefSeq	YP_190623.1. NC_006677.1.
3D structure databases
HSSP	HSSP built from PDB template 1WSG based on UniProtKB P0A7Y4.
ProteinModelPortal	Q5FUH9.
ModBase	Search...
Protein-protein interaction databases
STRING	290633.GOX0177.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW59967; AAW59967; GOX0177.
GeneID	3248733.
KEGG	gox:GOX0177.
PATRIC	32608030. VBIGluOxy81109_0402.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0328.
HOGENOM	HOG000040465.
KO	K03469.
OMA	MQEIEIF.
ProtClustDB	PRK00203.
Enzyme and pathway databases
BioCyc	GOXY290633:GHB3-235-MONOMER.
Family and domain databases
HAMAP	MF_00042. RNase_H.
InterPro	IPR022892. RNaseH. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. [Graphical view]
Pfam	PF00075. RNase_H. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50879. RNASE_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH_GLUOX

Accession

Primary (citable) accession number: Q5FUH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program