Reviewed, UniProtKB/Swiss-Prot Q5FRS2 (HUTI_GLUOX)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	GOX1163

Organism

Gluconobacter oxydans (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Imidazolonepropionase HAMAP MF_00372		PRO_0000306463
Sites
	Metal binding	76	1	Zinc or iron By similarity
	Metal binding	78	1	Zinc or iron By similarity
	Metal binding	246	1	Zinc or iron By similarity
	Metal binding	321	1	Zinc or iron By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FRS2-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 04873235E2A0DC4C	FASTA	412	45,076
	10 20 30 40 50 60 MWDTLWTDIH VATADPALIS DQDGYGCIHN AAIGIENGRI SWIGAARDLP DAPQNLAHKI 70 80 90 100 110 120 HSGNGLWMTP GLVDAHTHVI YAGDRSLEFA ERLNGVSYEE IARKGGGIIS TVNATRHVSE 130 140 150 160 170 180 DELFDVTARR VRRMIAEGTT TLEMKSGYGL TLQDELKQLR VARAIGTALP VRVHATFLGA 190 200 210 220 230 240 HALPSEFKDR QDDYVSHLIT DILPQAVEEK LIDSIDGFCE NIAFTPDQIE RLFIAANDLK 250 260 270 280 290 300 IPVRLHSEQL SNSGGTKLAA RYGALSTDHL EYVVEDDVID LAHAGTVAML LPGAFYFIRE 310 320 330 340 350 360 TRMPPVDLFR KHNVPMGLAT DCNPGTSPSV SLTGIMNMAC TLFRMTPEET FSAVTRIGAQ 370 380 390 400 410 ALGLQNVTGS LTTGQTADML LWDLAGPHEL SYWIGGRRPV QRVFDGKLNP VI

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000009 Genomic DNA. Translation: AAW60924.1.
RefSeq	YP_191580.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3249738.
GenomeReviews	Gene locus GOX1163 in contig CP000009_GR.
KEGG	gox:GOX1163.
NMPDR	fig|290633.1.peg.1125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5FRS2.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX1163-MON.
BRENDA	3.5.2.7. 3050.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_GLUOX

Accession

Primary (citable) accession number: Q5FRS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)