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Q5FRI6

- SYI_GLUOX

UniProt

Q5FRI6 - SYI_GLUOX

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Protein
Isoleucine--tRNA ligase
Gene
ileS, GOX1249
Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061Aminoacyl-adenylate By similarity
Binding sitei650 – 6501ATP By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi934 – 9341Zinc By similarity
Metal bindingi951 – 9511Zinc By similarity
Metal bindingi954 – 9541Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-1247-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:GOX1249
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
ProteomesiUP000006375: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098393Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290633.GOX1249.

Structurei

3D structure databases

ProteinModelPortaliQ5FRI6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 9211"HIGH" regionUniRule annotation
Add
BLAST
Motifi647 – 6515"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FRI6-1 [UniParc]FASTAAdd to Basket

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MTETGSSLMS DKTPDPSVAA FKEQQDRYRD TVFLPRTGFP MRGGLPKREP    50
ETLARWAATG LDEKIREAGK GRPVFTLHDG PPYANGHIHI GHALNKVMKD 100
VINRAHRMSG YEVRYLPGWD CHGLPIEWRI EEQYRKAGKD KDQVPLLQFR 150
AECRQYAAQW VQKQAEDFKR IGVQAEWANR YVTMDFSSEA KIVDEIGKFL 200
LNGSLYRGLR PVMWSPVEKT ALAEAEIEYH DIDSTTIFVA FPIIKDPTPA 250
HALNGVSAVI WTTTPWTIPA NRALAYGPDI TYVVLRVDET NENSVVPQGA 300
KLLVAEDRVD AFCTETGITA HHILYTLPGN GLEGAVCAHP LRGRGYEYDV 350
PMLPGDFVTT DAGTGLVHMA PSHGQDDFML CRQYGIEVPE LVQDDGRYAP 400
WVPHLAGIHV FKAADPVCDL LTEARQSAEH DDAPATGLMA RGTVRHSYPH 450
SWRSKAPIIF RATPQWFIAM DGETKLREKS LSALDNVTFV PEAARRRLTS 500
MIEQRPDWCI SRQRAWGVPI AVFVEKRTGE VLRDAAVMQR IVEAFREKGA 550
DIWYEADPSL FLGEGRNPAD YEQVFDIVDV WFESGSTHRF DLGQEGLNFP 600
ADLYLEGSDQ HRGWFQSSLL ESVGTMGVAP YKALVTNGFV MDEQGRKMSK 650
SLGNVVAPSD VTESLGADIL RLWVLNSDTN EDLRIGQDIL KQQGELYRRL 700
RNTLRWLLGA LDGFTPEEAV SYADLPPLEQ YILHRLTELR GLIASAVETH 750
QWVGVYPALH GFCTTDLSAF YFDIRKDAIY CDAPSDPTRR AARTVLDALH 800
RALCTWLAPV LVFTAEEAWQ ARFGEDDSVH LAQFFEPEAE WNDPELGNRW 850
EDIRAYRRLI TTELETARRD GTIGSSLEAS VTLPLSQEEA GIFGGLDWAE 900
LLITSHAETE LLPGDTAHGG PQVERAPGHK CARCWKVLPE VGENTEHPAL 950
CRRCIDVVSA 960
Length:960
Mass (Da):107,557
Last modified:March 1, 2005 - v1
Checksum:i8492209569DE222A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000009 Genomic DNA. Translation: AAW61010.1.
RefSeqiYP_191666.1. NC_006677.1.

Genome annotation databases

EnsemblBacteriaiAAW61010; AAW61010; GOX1249.
GeneIDi3250712.
KEGGigox:GOX1249.
PATRICi32610298. VBIGluOxy81109_1510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000009 Genomic DNA. Translation: AAW61010.1 .
RefSeqi YP_191666.1. NC_006677.1.

3D structure databases

ProteinModelPortali Q5FRI6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 290633.GOX1249.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW61010 ; AAW61010 ; GOX1249 .
GeneIDi 3250712.
KEGGi gox:GOX1249.
PATRICi 32610298. VBIGluOxy81109_1510.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci GOXY290633:GHB3-1247-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
    Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
    Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 621H.

Entry informationi

Entry nameiSYI_GLUOX
AccessioniPrimary (citable) accession number: Q5FRI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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