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Q5FRI6

- SYI_GLUOX

UniProt

Q5FRI6 - SYI_GLUOX

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei606 – 6061Aminoacyl-adenylateUniRule annotation
    Binding sitei650 – 6501ATPUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation
    Metal bindingi934 – 9341ZincUniRule annotation
    Metal bindingi951 – 9511ZincUniRule annotation
    Metal bindingi954 – 9541ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciGOXY290633:GHB3-1247-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:GOX1249
    OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
    Taxonomic identifieri290633 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
    ProteomesiUP000006375: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 960960Isoleucine--tRNA ligasePRO_0000098393Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290633.GOX1249.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FRI6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi82 – 9211"HIGH" regionAdd
    BLAST
    Motifi647 – 6515"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5FRI6-1 [UniParc]FASTAAdd to Basket

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    MTETGSSLMS DKTPDPSVAA FKEQQDRYRD TVFLPRTGFP MRGGLPKREP    50
    ETLARWAATG LDEKIREAGK GRPVFTLHDG PPYANGHIHI GHALNKVMKD 100
    VINRAHRMSG YEVRYLPGWD CHGLPIEWRI EEQYRKAGKD KDQVPLLQFR 150
    AECRQYAAQW VQKQAEDFKR IGVQAEWANR YVTMDFSSEA KIVDEIGKFL 200
    LNGSLYRGLR PVMWSPVEKT ALAEAEIEYH DIDSTTIFVA FPIIKDPTPA 250
    HALNGVSAVI WTTTPWTIPA NRALAYGPDI TYVVLRVDET NENSVVPQGA 300
    KLLVAEDRVD AFCTETGITA HHILYTLPGN GLEGAVCAHP LRGRGYEYDV 350
    PMLPGDFVTT DAGTGLVHMA PSHGQDDFML CRQYGIEVPE LVQDDGRYAP 400
    WVPHLAGIHV FKAADPVCDL LTEARQSAEH DDAPATGLMA RGTVRHSYPH 450
    SWRSKAPIIF RATPQWFIAM DGETKLREKS LSALDNVTFV PEAARRRLTS 500
    MIEQRPDWCI SRQRAWGVPI AVFVEKRTGE VLRDAAVMQR IVEAFREKGA 550
    DIWYEADPSL FLGEGRNPAD YEQVFDIVDV WFESGSTHRF DLGQEGLNFP 600
    ADLYLEGSDQ HRGWFQSSLL ESVGTMGVAP YKALVTNGFV MDEQGRKMSK 650
    SLGNVVAPSD VTESLGADIL RLWVLNSDTN EDLRIGQDIL KQQGELYRRL 700
    RNTLRWLLGA LDGFTPEEAV SYADLPPLEQ YILHRLTELR GLIASAVETH 750
    QWVGVYPALH GFCTTDLSAF YFDIRKDAIY CDAPSDPTRR AARTVLDALH 800
    RALCTWLAPV LVFTAEEAWQ ARFGEDDSVH LAQFFEPEAE WNDPELGNRW 850
    EDIRAYRRLI TTELETARRD GTIGSSLEAS VTLPLSQEEA GIFGGLDWAE 900
    LLITSHAETE LLPGDTAHGG PQVERAPGHK CARCWKVLPE VGENTEHPAL 950
    CRRCIDVVSA 960
    Length:960
    Mass (Da):107,557
    Last modified:March 1, 2005 - v1
    Checksum:i8492209569DE222A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000009 Genomic DNA. Translation: AAW61010.1.
    RefSeqiYP_191666.1. NC_006677.1.

    Genome annotation databases

    EnsemblBacteriaiAAW61010; AAW61010; GOX1249.
    GeneIDi3250712.
    KEGGigox:GOX1249.
    PATRICi32610298. VBIGluOxy81109_1510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000009 Genomic DNA. Translation: AAW61010.1 .
    RefSeqi YP_191666.1. NC_006677.1.

    3D structure databases

    ProteinModelPortali Q5FRI6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290633.GOX1249.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW61010 ; AAW61010 ; GOX1249 .
    GeneIDi 3250712.
    KEGGi gox:GOX1249.
    PATRICi 32610298. VBIGluOxy81109_1510.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci GOXY290633:GHB3-1247-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
      Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
      Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 621H.

    Entry informationi

    Entry nameiSYI_GLUOX
    AccessioniPrimary (citable) accession number: Q5FRI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3