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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061Aminoacyl-adenylateUniRule annotation
Binding sitei650 – 6501ATPUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi934 – 9341ZincUniRule annotation
Metal bindingi951 – 9511ZincUniRule annotation
Metal bindingi954 – 9541ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-1247-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:GOX1249
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
ProteomesiUP000006375: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Isoleucine--tRNA ligasePRO_0000098393Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290633.GOX1249.

Structurei

3D structure databases

ProteinModelPortaliQ5FRI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 9211"HIGH" regionAdd
BLAST
Motifi647 – 6515"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FRI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETGSSLMS DKTPDPSVAA FKEQQDRYRD TVFLPRTGFP MRGGLPKREP
60 70 80 90 100
ETLARWAATG LDEKIREAGK GRPVFTLHDG PPYANGHIHI GHALNKVMKD
110 120 130 140 150
VINRAHRMSG YEVRYLPGWD CHGLPIEWRI EEQYRKAGKD KDQVPLLQFR
160 170 180 190 200
AECRQYAAQW VQKQAEDFKR IGVQAEWANR YVTMDFSSEA KIVDEIGKFL
210 220 230 240 250
LNGSLYRGLR PVMWSPVEKT ALAEAEIEYH DIDSTTIFVA FPIIKDPTPA
260 270 280 290 300
HALNGVSAVI WTTTPWTIPA NRALAYGPDI TYVVLRVDET NENSVVPQGA
310 320 330 340 350
KLLVAEDRVD AFCTETGITA HHILYTLPGN GLEGAVCAHP LRGRGYEYDV
360 370 380 390 400
PMLPGDFVTT DAGTGLVHMA PSHGQDDFML CRQYGIEVPE LVQDDGRYAP
410 420 430 440 450
WVPHLAGIHV FKAADPVCDL LTEARQSAEH DDAPATGLMA RGTVRHSYPH
460 470 480 490 500
SWRSKAPIIF RATPQWFIAM DGETKLREKS LSALDNVTFV PEAARRRLTS
510 520 530 540 550
MIEQRPDWCI SRQRAWGVPI AVFVEKRTGE VLRDAAVMQR IVEAFREKGA
560 570 580 590 600
DIWYEADPSL FLGEGRNPAD YEQVFDIVDV WFESGSTHRF DLGQEGLNFP
610 620 630 640 650
ADLYLEGSDQ HRGWFQSSLL ESVGTMGVAP YKALVTNGFV MDEQGRKMSK
660 670 680 690 700
SLGNVVAPSD VTESLGADIL RLWVLNSDTN EDLRIGQDIL KQQGELYRRL
710 720 730 740 750
RNTLRWLLGA LDGFTPEEAV SYADLPPLEQ YILHRLTELR GLIASAVETH
760 770 780 790 800
QWVGVYPALH GFCTTDLSAF YFDIRKDAIY CDAPSDPTRR AARTVLDALH
810 820 830 840 850
RALCTWLAPV LVFTAEEAWQ ARFGEDDSVH LAQFFEPEAE WNDPELGNRW
860 870 880 890 900
EDIRAYRRLI TTELETARRD GTIGSSLEAS VTLPLSQEEA GIFGGLDWAE
910 920 930 940 950
LLITSHAETE LLPGDTAHGG PQVERAPGHK CARCWKVLPE VGENTEHPAL
960
CRRCIDVVSA
Length:960
Mass (Da):107,557
Last modified:March 1, 2005 - v1
Checksum:i8492209569DE222A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW61010.1.
RefSeqiWP_011252802.1. NC_006677.1.
YP_191666.1. NC_006677.1.

Genome annotation databases

EnsemblBacteriaiAAW61010; AAW61010; GOX1249.
GeneIDi3250712.
KEGGigox:GOX1249.
PATRICi32610298. VBIGluOxy81109_1510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW61010.1.
RefSeqiWP_011252802.1. NC_006677.1.
YP_191666.1. NC_006677.1.

3D structure databases

ProteinModelPortaliQ5FRI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290633.GOX1249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW61010; AAW61010; GOX1249.
GeneIDi3250712.
KEGGigox:GOX1249.
PATRICi32610298. VBIGluOxy81109_1510.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-1247-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
    Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
    Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 621H.

Entry informationi

Entry nameiSYI_GLUOX
AccessioniPrimary (citable) accession number: Q5FRI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: January 7, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.