Q5FRG7 (SYE1_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	GOX1268

Organism

Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

290633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000119569
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FRG7 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 60C7FF557365D4E0	FASTA	472	52,303
	10 20 30 40 50 60 MTIRTRFAPS PTGLLHVGNA RAALFNFLFA RHHGGEFLLR IEDTDKERST QKAVDVIFDG 70 80 90 100 110 120 LAWMGIEADA EPVFQSARQD RHTEVALELL AKGQAYKCFC TPEELTAMRE KAMAEKRPPR 130 140 150 160 170 180 YDGTWRDRDP SEAPEGAPYV VRLKAPREGE TVIKDLVQGE VRVANAEMDD LILLRSDGTP 190 200 210 220 230 240 TYLHAVVCDD HDMEITHVMR GDDHLTNTFR QAMIYRAMGW DLPHFAHLPL IHGPDGAKLS 250 260 270 280 290 300 KRHGAQSVVD FREEGYLPEA LCNYLLRLGW GHGDAEVLSR EEQIKLFDLD GVGRSPSRMD 310 320 330 340 350 360 YVKLAHLNGI WMRQADDERL TNDVMERLQG REGVVTDDKT RARILAMMPG LKERAKTLVE 370 380 390 400 410 420 LADNAAFAGF TLPLTFTPKA EKLLGEDGRR VLEGVGKELA ALKDFTPEAI DATLRSYAEK 430 440 450 460 470 HEIKLGSVAQ PLRAAMTGST TSPGIDLTLS ALGQDEVLAR IAAVLASGSA NA

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000009 Genomic DNA. Translation: AAW61029.1.
RefSeq	YP_191685.1. NC_006677.1.
3D structure databases
ProteinModelPortal	Q5FRG7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3250731.
GenomeReviews	Gene locus GOX1268 in contig CP000009_GR.
KEGG	gox:GOX1268.
NMPDR	fig|290633.1.peg.1230.
PATRIC	32610336. VBIGluOxy81109_1529.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX1268-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_GLUOX

Accession

Primary (citable) accession number: Q5FRG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program