Q5FQP6 (ISPE_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	GOX1559

Organism

Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

290633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235096
Regions
	Nucleotide binding	110 – 120	11	ATP Potential
Sites
	Active site	19	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FQP6 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 3E5AC62EA70DC5E3	FASTA	309	31,929
	10 20 30 40 50 60 MTFPDTTSSA APQATAHAKI NLFLHITGRR DDGYHLLDSL AVFAGAADRV TLTAPSNTMS 70 80 90 100 110 120 LTIDGPFGQG LEADDQNLVL RAARALQAQA GTTIGGLTES FHLELQKNLP VSSGIGGGSA 130 140 150 160 170 180 DSAATLRLLA REWNVDQAAL FPIAETLGAD VPVCVAQRPT RMQGIGEILA PAPVLPDAGL 190 200 210 220 230 240 LLVNPGVGVS TPDVFRAFAA GGGIVERPEP FLPDRWNTAQ DMVADLNATT NDLQAPALSQ 250 260 270 280 290 300 CPVIGTVLAS IESLPGCLLS RMSGSGATCF GIFSSPESAV AAAHVLNAQS ARLGWWTWAG GFHHTGAST

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000009 Genomic DNA. Translation: AAW61300.1.
RefSeq	YP_191956.1. NC_006677.1.
3D structure databases
ProteinModelPortal	Q5FQP6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3250191.
GenomeReviews	Gene locus GOX1559 in contig CP000009_GR.
KEGG	gox:GOX1559.
NMPDR	fig|290633.1.peg.1501.
PATRIC	32610964. VBIGluOxy81109_1823.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	CQLARMS.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX1559-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_GLUOX

Accession

Primary (citable) accession number: Q5FQP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program