Q5FQ35 (SYE2_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	GOX1772

Organism

Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

290633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000119570
Regions
	Motif	7 – 17	11	"HIGH" region HAMAP MF_00022_B
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FQ35 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 991485C62FB6431E	FASTA	444	49,905
	10 20 30 40 50 60 MKLRFAPSPT GYLHVGNARL AVANFLFARH NGAKFLLRID DTDTTRGKPE YEEAIGKDLS 70 80 90 100 110 120 WLGLKWDEYV RQSERLDRYA EVIEKLKASG RLYPCFETEY ELNAKREARI RAGKAPIYDR 130 140 150 160 170 180 AMLKLTADQR ARAEANGKTP HWRFRLSDGS RKWQDLVMDE CSVKLTAISD PVLVRGDGTI 190 200 210 220 230 240 LYTLASVIDD LDMGITHIVR GEDHVTNTGV QIDIAEALGA KPDHFTFAHL PLLLDSDGGK 250 260 270 280 290 300 LSKRFDSLAL KSLRQDGLEP MSIVSYLARV GSSDDPQVMT MDEAIAAYDI SHVSKSAARF 310 320 330 340 350 360 DMTQLLALNR RALHNLPFED AKIHLPPEAD ETFWHAVRGN VDLSAEIPHW WDVVHGTIIP 370 380 390 400 410 420 PSQPEDRAFL QHALDTLPAE PWGEETWKDW TNALKEQSGR KGRSLFMPLR LALTGEDAGP 430 440 ELHVLLGLMG RERTVARLRD AIQA

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000009 Genomic DNA. Translation: AAW61511.1.
RefSeq	YP_192167.1. NC_006677.1.
3D structure databases
ProteinModelPortal	Q5FQ35.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3250036.
GenomeReviews	Gene locus GOX1772 in contig CP000009_GR.
KEGG	gox:GOX1772.
NMPDR	fig|290633.1.peg.1712.
PATRIC	32611430. VBIGluOxy81109_2054.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX1772-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_GLUOX

Accession

Primary (citable) accession number: Q5FQ35

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program