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Q5FPT6

- GLND_GLUOX

UniProt

Q5FPT6 - GLND_GLUOX

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-1870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:GOX1872
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
ProteomesiUP000006375: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192735Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290633.GOX1872.

Structurei

3D structure databases

ProteinModelPortaliQ5FPT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini541 – 62989HDUniRule annotationAdd
BLAST
Domaini757 – 83478ACT 1UniRule annotationAdd
BLAST
Domaini870 – 94980ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 395395UridylyltransferaseAdd
BLAST
Regioni396 – 756361Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FPT6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKETSFWGET PSLSFADDTD KPLSDRTASP PCDPASSLQT ALDTAAAQGQ
60 70 80 90 100
TTRENVLSIL RRHLGSGNAT VRREFEKRRM SGIDAARALA RQADDMVCAL
110 120 130 140 150
AELAAQKHES PGETLCLCAT GGYGAGLLAP FSDIDILFLI PGDPTPAMTA
160 170 180 190 200
RIEFILYALW DLGLRVGHAT RSIAECVRDA DSDLTIRTAL LDLRFLHGER
210 220 230 240 250
GLARDLRCAL GADLQNDRLC EFVMGKIAER EQRHRRFGDN PYMVEPNIKE
260 270 280 290 300
GRGGLRDLQT LNWMGRAALG CAVSTPDRSG QPAEAPQTPS FASFGLLTDR
310 320 330 340 350
ESLRARRSWD FLWTVRLHLH YITGRAEERL TFDVQPVIGG RMGYATHGRQ
360 370 380 390 400
RGVERFMRHY FLTARDVMRL TSVLQPVVLM HLQDQTTGEP PKVVPGPEEF
410 420 430 440 450
QTIAGRICPI EPVTFAAQPR EMFRLLDCGR RHDLPLHPIA MQQIIRNERH
460 470 480 490 500
AVTLRDDPET AKIFLDLLCE PSADETKAVP FWLPILNETG LLGRLLPDWS
510 520 530 540 550
RVVGQMQFDS YHIYTVDEHI VEAVRMMGQI EAGRMADEIP LAYTLASDLR
560 570 580 590 600
SRRALYVAVL LHDIGKGRGG DHSEIGADLA LTICPQLGLD PEETDTVSWL
610 620 630 640 650
VLHHLLLSQT AFTRDIDDPR TILDLADTIQ SPERLRLLLL LTIADMRAVS
660 670 680 690 700
PKVWNAWKAT LLRELFSRVA EVLEGGLAAT ERDSRVNHAR ELARDGLTGI
710 720 730 740 750
LPESSIDRFL DLGYPSYWLG FDTDTQMRHA RMVHDSDRYR SPVTVEAYPI
760 770 780 790 800
PERGVTELTV LCADHPGLFS QIAGALAVSG ASIVDARIHT LSDGMALDTF
810 820 830 840 850
WVQDGEGCSF EEPHQLGRLN HLVEQALSGR LDIRKGIEDA SHHSTSRRMR
860 870 880 890 900
AIHVPPRVVI DNTASDRHTV IEVNGRDRPG LLHDVTSALS SASLQISSAH
910 920 930 940
ITTYGMRAVD VFYVRDLLGM KITDPVRLAR LRETLLASLT SAPVTTPAS
Length:949
Mass (Da):105,398
Last modified:March 1, 2005 - v1
Checksum:i79BEEE7485522A9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW61610.1.
RefSeqiYP_192266.1. NC_006677.1.

Genome annotation databases

EnsemblBacteriaiAAW61610; AAW61610; GOX1872.
GeneIDi3250631.
KEGGigox:GOX1872.
PATRICi32611646. VBIGluOxy81109_2161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW61610.1 .
RefSeqi YP_192266.1. NC_006677.1.

3D structure databases

ProteinModelPortali Q5FPT6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290633.GOX1872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW61610 ; AAW61610 ; GOX1872 .
GeneIDi 3250631.
KEGGi gox:GOX1872.
PATRICi 32611646. VBIGluOxy81109_2161.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci GOXY290633:GHB3-1870-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans."
    Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.
    Nat. Biotechnol. 23:195-200(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 621H.

Entry informationi

Entry nameiGLND_GLUOX
AccessioniPrimary (citable) accession number: Q5FPT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2005
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3