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Q5FMN6 (DDL_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LBA0137
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341115

Regions

Domain134 – 343210ATP-grasp
Nucleotide binding169 – 22456ATP By similarity

Sites

Metal binding2971Magnesium or manganese 1 By similarity
Metal binding3101Magnesium or manganese 1 By similarity
Metal binding3101Magnesium or manganese 2 By similarity
Metal binding3121Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FMN6 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 5DD3A3DF2D2D1787

FASTA36040,246
        10         20         30         40         50         60 
MTKKIQVGLI FGGNSSEYEV SIVSGHNIYK AIDKDKFDVH PIWITNEGYF ASEEESFKVL 

        70         80         90        100        110        120 
EDPSYQVENP HKVNNISNII ELKNLPEIDV FFPIVHGNLG EDGVLQGLFR LMNKPFVGDD 

       130        140        150        160        170        180 
VLAAAATMDK EFTKILAQRV GVPVADWISI KRFEYDDKNN DKLNYEKVAE KLGHDMFVKP 

       190        200        210        220        230        240 
SNQGSSVGVN HVTNAEEYAA ALEEAFKYDD KVLVEETVPG TEVETAVLGN DKPIVAGVGQ 

       250        260        270        280        290        300 
ITNAKGSFYT YENKYDDNST SKLQIPADLP QDIVDTVREN ARKVYEITEC SGMARIDSML 

       310        320        330        340        350        360 
TPDGKVVLTE VNALPGFTNI SMYPKLFEEA GIPYTELITR LIQAGMDRFD HKKTLLHKHD 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV42038.1.
RefSeqYP_193069.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FMN6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA0137.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV42038; AAV42038; LBA0137.
GeneID3252845.
KEGGlac:LBA0137.
PATRIC22195327. VBILacAci7974_0133.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LACAC
AccessionPrimary (citable) accession number: Q5FMN6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways