Q5FMB2 (ALR_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	LBA0269

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alanine racemase HAMAP MF_01201		PRO_1000065995
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	270	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FMB2 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 7701DC865AEC3E9F	FASTA	376	41,600
	10 20 30 40 50 60 MVPGYHRPAA VKVNLGAIRR NLENEMKHLD PGQKMLAVVK ANAYGHGAVE VAKVAEEVGA 70 80 90 100 110 120 AGFCVAVLDE GLQLRHADIV KPILVLGVVS PKYAPIAAVN NISLTVPNFE WLKEAEKYLA 130 140 150 160 170 180 KENLQLKIHL GIDSGMGRIG FNEDDEFIEA NKFLENNDQF FVEGMFAHFA SADSADESYF 190 200 210 220 230 240 EHQLEKFNHM KSLLTVKPKW IHVSNTAASI FHKNIKSDLV RFGIGIYGLN PSSNPASADL 250 260 270 280 290 300 NPDIKLEPAL SFESELTHVK TIHKGDGVSY GSTFVADKDT IIGTVPVGYA DGWIRKFQGF 310 320 330 340 350 360 KVKVGDKYCP IVGRICMDQF MVELPEKMPV GTKVVIISNN PDDPNNIKAA ADYVNTIHYE 370 VACLLNDRLP RIYYEK

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42162.1.
RefSeq	YP_193193.1. NC_006814.3.
3D structure databases
HSSP	HSSP built from PDB template 1NIU based on UniProtKB P10724.
ProteinModelPortal	Q5FMB2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3252362.
GenomeReviews	Gene locus LBA0269 in contig CP000033_GR.
KEGG	lac:LBA0269.
NMPDR	fig|272621.3.peg.308.
PATRIC	22195573. VBILacAci7974_0254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	TRKEDAN.
ProtClustDB	CLSK925011.
Enzyme and pathway databases
BioCyc	LACI272621:LBA0269-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LACAC

Accession

Primary (citable) accession number: Q5FMB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program