Reviewed, UniProtKB/Swiss-Prot Q5FMB0 (LDH1_LACAC)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldh1
Ordered Locus Names:	LBA0271

Organism

Lactobacillus acidophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000237547
Regions
	Nucleotide binding	16 – 44	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor HAMAP MF_00488
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FMB0-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: DD2B0FFBC2ADA228	FASTA	323	35,039
	10 20 30 40 50 60 MARVEKPRKV ILVGDGAVGS TFAFSMVQQG IAEELGIIDI AKEHVEGDAI DLADATPWTF 70 80 90 100 110 120 PKNIYAADYA DCKDADLVVI TAGAPQKPGE TRLDLVNKNL KILSSIVEPV VESGFEGIFL 130 140 150 160 170 180 VVANPVDILT HATWKISGFP KDRVIGSGTS LDTGRLQKVI GKMEHVDPRS VNAYMLGEHG 190 200 210 220 230 240 DTEFPVWSYN NVGGVKVSDW VKAHGMDESK LEEIHKEVAD MAYDIINKKG ATFYGIGTAS 250 260 270 280 290 300 AMIAKAILND EHRVLPLSVA MDGQYGLHDL HIGTPAVVGR NGLEQIIEMP LTADEQAKME 310 320 ASAKQLKEVM DKAFEETGVK VRQ

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCFM/NCK56/N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42164.1.
RefSeq	YP_193195.1.
3D structure databases
SMR	Q5FMB0. Positions 8-314.
ModBase	Search...
Genome annotation databases
GeneID	3252415.
GenomeReviews	Gene locus LBA0271 in contig CP000033_GR.
KEGG	lac:LBA0271.
NMPDR	fig|272621.3.peg.310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	CAGANQK.
Enzyme and pathway databases
BioCyc	LACI272621:LBA0271-MONOMER.
BRENDA	1.1.1.27. 3045.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_LACAC

Accession

Primary (citable) accession number: Q5FMB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)