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Protein

dITP/XTP pyrophosphatase

Gene

LBA0426

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Proton acceptorUniRule annotation1
Metal bindingi73MagnesiumUniRule annotation1
Binding sitei74Substrate; via amide nitrogenUniRule annotation1
Binding sitei181SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLACI272621:GJO8-415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:LBA0426
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000006381 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001781771 – 202dITP/XTP pyrophosphataseAdd BLAST202

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272621.LBA0426.

Structurei

3D structure databases

ProteinModelPortaliQ5FLV7.
SMRiQ5FLV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 14Substrate bindingUniRule annotation6
Regioni158 – 161Substrate bindingUniRule annotation4
Regioni186 – 187Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5FLV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKILFATG NKGKARELKE AFKTAGVDVE IITNSDLDNP PHPIESGRTF
60 70 80 90 100
EANAKIKAHE LADYSKLPTI ADDSGLMVDA LNGEPGVRSA RYAGEAHNDA
110 120 130 140 150
KNNAKLLANL GGIPDEKRTA KFWTTIVVSM PGEFEKDLVV SGTCSGRILA
160 170 180 190 200
APRGDDGFGY DPLFFVPKKD KTFAQMTTDE KNEISHRGNV VRELLKVLPA

LA
Length:202
Mass (Da):21,884
Last modified:March 1, 2005 - v1
Checksum:i8DBF096BAB41C4E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV42317.1.
RefSeqiWP_011254149.1. NC_006814.3.
YP_193348.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV42317; AAV42317; LBA0426.
GeneIDi3252582.
KEGGilac:LBA0426.
PATRICi22195894. VBILacAci7974_0411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV42317.1.
RefSeqiWP_011254149.1. NC_006814.3.
YP_193348.1. NC_006814.3.

3D structure databases

ProteinModelPortaliQ5FLV7.
SMRiQ5FLV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272621.LBA0426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV42317; AAV42317; LBA0426.
GeneIDi3252582.
KEGGilac:LBA0426.
PATRICi22195894. VBILacAci7974_0411.

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Enzyme and pathway databases

BioCyciLACI272621:GJO8-415-MONOMER.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIXTPA_LACAC
AccessioniPrimary (citable) accession number: Q5FLV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2005
Last modified: April 12, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.