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Q5FL68 (GLGB_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:LBA0680
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6386381,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188711

Sites

Active site3031Nucleophile By similarity
Active site3561Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FL68 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: F66FC75355225AAC

FASTA63874,582
        10         20         30         40         50         60 
MVTIVEVKQH IKKFAAGNEL YLQEVLGCHY ENDIYTFRVW APNAQKVWLV GDFNDWDKSL 

        70         80         90        100        110        120 
EMSQTLDGVW EIKTSLPKEG QLYKFLVKQA DGREVMKIDP MAFELEPRPG SAAVIVKLPN 

       130        140        150        160        170        180 
KKWLDGAWMG RNKRSNHFAR PINIYEVHAS SWKRHTDGSL YTLKDLQKEL IPYVKEQGFN 

       190        200        210        220        230        240 
YIEFLPLTAH PLDASWGYQT IGYYALERTY GTPRELQDFV EACHKENIGV LADWVPGHFC 

       250        260        270        280        290        300 
INDDALAYYD GTPCYEFSEK WRAENKGWGA LNFDLGKPEV QSFLLSSALF WLEFYHLDGL 

       310        320        330        340        350        360 
RVDAVSNMIY RDYDRSDGEW KTDKFGGNRN LEGIEFLQKL NRTIKGKHPE CLMIAEESSA 

       370        380        390        400        410        420 
QVKITGRIED GGLGFDFKWN MGWMNDILRF YEMDPLFRKF NFNLATFSFM YRMSENFILP 

       430        440        450        460        470        480 
LSHDEVVHGK RSLMNKMFGD RDKQFAQLRN LLTLQMTYPG KKLLFMGSEF GQYLEWRYND 

       490        500        510        520        530        540 
GLDWAELKDE LNAKMKHFDQ DLNSFYLNEP ALWQLEQRED SVQIIDADNK DESVLSFIRQ 

       550        560        570        580        590        600 
GKTRHDFLIV ILNFTPVDRK KITIGVPYAG KYCEVFNSAR KEYGGSWNQE KQNLKTQNNS 

       610        620        630 
FKNFNYQVQL DIPGFSAVIL KPVDVHIKRR INRKTKTK 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV42556.1.
RefSeqYP_193587.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FL68.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA0680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV42556; AAV42556; LBA0680.
GeneID3251253.
KEGGlac:LBA0680.
PATRIC22196464. VBILacAci7974_0650.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_LACAC
AccessionPrimary (citable) accession number: Q5FL68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries