Q5FL41 (POTA_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	LBA0709

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus acidophilus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286226
Regions
	Domain	4 – 235	232	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5FL41 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 0637AF69C30F7B79	FASTA	365	41,156
	10 20 30 40 50 60 MDIIKLKHIT KRYDDGFIAL KDINITIESG KFYSLLGPSG SGKSTILRII AGFTQPSSGQ 70 80 90 100 110 120 VFFDGKDITD LDASKRHINT VFQNYALFPH LNVFENVAFG LKIKKKPLNE IRPAVKDALH 130 140 150 160 170 180 MVRLDGYANR EISELSGGQQ QRVAIARAIV NEPKVLLLDE SLSALDKRLR KDMQFELRAI 190 200 210 220 230 240 QKKLGITFIF VTHDQEEALS MSDEIFVLND GQIQQSGSPV AIYDEPVNDF VARFIGDSNI 250 260 270 280 290 300 LPGRMIKDYV VEFANNKFRC ADGGIKSNEK VEVVIRPEDL DIVAPEKGKI VVTAQTQLFL 310 320 330 340 350 360 GDHFEIKAID ANENEWLIHS TNGVKLGQRI GLFFDPEDIH VMRLNESQKD FDARIEKYEG DENED

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42583.1.
RefSeq	YP_193614.1. NC_006814.3.
3D structure databases
ProteinModelPortal	Q5FL41.
ModBase	Search...
Protein-protein interaction databases
STRING	272621.LBA0709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV42583; AAV42583; LBA0709.
GeneID	3251329.
KEGG	lac:LBA0709.
PATRIC	22196522. VBILacAci7974_0678.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	MELKEMH.
ProtClustDB	CLSK925162.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR027417. P-loop_NTPase. IPR005893. Sp_pt_ABC_ATP-bd. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01187. potA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_LACAC

Accession

Primary (citable) accession number: Q5FL41

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 1, 2005
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program