Q5FKZ1 (KITH_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	LBA0766

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus acidophilus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence AAV42633.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174981
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	93 – 96	4	ATP By similarity
Sites
	Active site	94	1	Proton acceptor Potential
	Metal binding	151	1	Zinc By similarity
	Metal binding	154	1	Zinc By similarity
	Metal binding	188	1	Zinc By similarity
	Metal binding	191	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FKZ1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: CEF52A5C50AADE36	FASTA	199	22,659
	10 20 30 40 50 60 MAQLFFRYGA MSSGKTIEIL KVAHNYEAQG RKIALMTSGL DNRSGVGTVA SRIGIHRKAI 70 80 90 100 110 120 PITEEMDLFE YIKEMNNHDL ADGDGKLACV LIDEAQFLKR HHVLECAKIV DEFNIPVMTF 130 140 150 160 170 180 GLKNDFQNHL FEGSENLLIF ADKIEEMKTI CHYCGHKATM NLRINNGKPV YEGEQVQIGG 190 DESYYPVCRY HYFHPNVVR

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42633.1. Different initiation.
RefSeq	YP_193664.1. NC_006814.3.
3D structure databases
ProteinModelPortal	Q5FKZ1.
ModBase	Search...
Protein-protein interaction databases
STRING	272621.LBA0766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV42633; AAV42633; LBA0766.
GeneID	3251539.
KEGG	lac:LBA0766.
PATRIC	22196628. VBILacAci7974_0729.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	LACI272621:GJO8-1264-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_LACAC

Accession

Primary (citable) accession number: Q5FKZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program