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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi886ZincUniRule annotation1
Metal bindingi889ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLACI272621:G1G49-829-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LBA0817
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000006381 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984011 – 927Isoleucine--tRNA ligaseAdd BLAST927

Proteomic databases

PRIDEiQ5FKU5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272621.LBA0817

Structurei

3D structure databases

ProteinModelPortaliQ5FKU5
SMRiQ5FKU5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q5FKU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIKDTLNLG KTKFKMRGNL PVREAEWEKE WEDNHLYEQR LKLNEGHPRF
60 70 80 90 100
DLHDGPPFAN GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP
110 120 130 140 150
VEQQLAKKGI DRKTMDRAKY RELCRQYAEE QVQKQMTDFK RLGVMADWDN
160 170 180 190 200
PYITLQHEFE GQEIRVFGEM YKKGYIYKGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
KDVEANSIFV AFPVVDSKGI IDPKDTYFVI WTTTPWTIPA NEAICVNPKF
260 270 280 290 300
DYSVVQVGDK KYVVATGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP
310 320 330 340 350
LYDKESLVTE GFHVTLDDGT GLVHTAPGFG ADDFNVGQKY DLPVFSPVDA
360 370 380 390 400
HGRYTDEVPE LEGMFYQDVD KLMVEKLKDA GALLKLKVFT HSYPHDWRTK
410 420 430 440 450
KPVIFRATTQ WFASIAPFRD QILEQIDNAK FIPSWGKTRL YNMIKDRGDW
460 470 480 490 500
VISRQRAWGV PLPIFYAEDG TPIVTPETIE HIAEIFDKEG SNAWYTHTAK
510 520 530 540 550
ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK RDGLHYPADL
560 570 580 590 600
YLEGSDQYRG WFNSSLITSV AVTGKAPYKE VLSQGFVLDD KGHKMSKSLG
610 620 630 640 650
NVISPNDVIK RMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT
660 670 680 690 700
FRYMLANTSD FDPKENGVAY DDLRSVDQYM EIKLNDLVAE CLAAYDKFDF
710 720 730 740 750
TTVFKKIFNF ISNDLSAFYL DFAKDVLYIE GKNSLERRSM QTVIYDAAVK
760 770 780 790 800
LTKILTPILP HTMEEIWGFL KEPEDYVQLA NMPKVENYTN HDELLENWGK
810 820 830 840 850
FMNLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM LDDLDADFRQ
860 870 880 890 900
ILIVSKLTIV DGEAPENAEK LNNASIVVEH AEGEVCPRCR MIRTDIGEDP
910 920
KLPELCERCA KIVEEDFPEA AQEGLEE
Length:927
Mass (Da):106,244
Last modified:March 1, 2005 - v1
Checksum:iF62F379E86F0D5E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA Translation: AAV42679.1
RefSeqiWP_011254260.1, NC_006814.3
YP_193710.1, NC_006814.3

Genome annotation databases

EnsemblBacteriaiAAV42679; AAV42679; LBA0817
GeneIDi3251646
KEGGilac:LBA0817
PATRICifig|272621.13.peg.779

Similar proteinsi

Entry informationi

Entry nameiSYI_LACAC
AccessioniPrimary (citable) accession number: Q5FKU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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