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Q5FKU5

- SYI_LACAC

UniProt

Q5FKU5 - SYI_LACAC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi886 – 8861ZincUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LBA0817
    OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
    Taxonomic identifieri272621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000006381: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 927927Isoleucine--tRNA ligasePRO_0000098401Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272621.LBA0817.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FKU5.
    SMRiQ5FKU5. Positions 1-915.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5FKU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIKDTLNLG KTKFKMRGNL PVREAEWEKE WEDNHLYEQR LKLNEGHPRF    50
    DLHDGPPFAN GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP 100
    VEQQLAKKGI DRKTMDRAKY RELCRQYAEE QVQKQMTDFK RLGVMADWDN 150
    PYITLQHEFE GQEIRVFGEM YKKGYIYKGK KPVYWSWSSE STLAEAEVEY 200
    KDVEANSIFV AFPVVDSKGI IDPKDTYFVI WTTTPWTIPA NEAICVNPKF 250
    DYSVVQVGDK KYVVATGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP 300
    LYDKESLVTE GFHVTLDDGT GLVHTAPGFG ADDFNVGQKY DLPVFSPVDA 350
    HGRYTDEVPE LEGMFYQDVD KLMVEKLKDA GALLKLKVFT HSYPHDWRTK 400
    KPVIFRATTQ WFASIAPFRD QILEQIDNAK FIPSWGKTRL YNMIKDRGDW 450
    VISRQRAWGV PLPIFYAEDG TPIVTPETIE HIAEIFDKEG SNAWYTHTAK 500
    ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK RDGLHYPADL 550
    YLEGSDQYRG WFNSSLITSV AVTGKAPYKE VLSQGFVLDD KGHKMSKSLG 600
    NVISPNDVIK RMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT 650
    FRYMLANTSD FDPKENGVAY DDLRSVDQYM EIKLNDLVAE CLAAYDKFDF 700
    TTVFKKIFNF ISNDLSAFYL DFAKDVLYIE GKNSLERRSM QTVIYDAAVK 750
    LTKILTPILP HTMEEIWGFL KEPEDYVQLA NMPKVENYTN HDELLENWGK 800
    FMNLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM LDDLDADFRQ 850
    ILIVSKLTIV DGEAPENAEK LNNASIVVEH AEGEVCPRCR MIRTDIGEDP 900
    KLPELCERCA KIVEEDFPEA AQEGLEE 927
    Length:927
    Mass (Da):106,244
    Last modified:March 1, 2005 - v1
    Checksum:iF62F379E86F0D5E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000033 Genomic DNA. Translation: AAV42679.1.
    RefSeqiYP_193710.1. NC_006814.3.

    Genome annotation databases

    EnsemblBacteriaiAAV42679; AAV42679; LBA0817.
    GeneIDi3251646.
    KEGGilac:LBA0817.
    PATRICi22196728. VBILacAci7974_0779.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000033 Genomic DNA. Translation: AAV42679.1 .
    RefSeqi YP_193710.1. NC_006814.3.

    3D structure databases

    ProteinModelPortali Q5FKU5.
    SMRi Q5FKU5. Positions 1-915.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272621.LBA0817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV42679 ; AAV42679 ; LBA0817 .
    GeneIDi 3251646.
    KEGGi lac:LBA0817.
    PATRICi 22196728. VBILacAci7974_0779.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700396 / NCK56 / N2 / NCFM.

    Entry informationi

    Entry nameiSYI_LACAC
    AccessioniPrimary (citable) accession number: Q5FKU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3