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Q5FKU5

- SYI_LACAC

UniProt

Q5FKU5 - SYI_LACAC

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Protein

Isoleucine--tRNA ligase

Gene
ileS, LBA0817
Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi886 – 8861Zinc By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LBA0817
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligaseUniRule annotationPRO_0000098401Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272621.LBA0817.

Structurei

3D structure databases

ProteinModelPortaliQ5FKU5.
SMRiQ5FKU5. Positions 1-915.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FKU5-1 [UniParc]FASTAAdd to Basket

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MRIKDTLNLG KTKFKMRGNL PVREAEWEKE WEDNHLYEQR LKLNEGHPRF    50
DLHDGPPFAN GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP 100
VEQQLAKKGI DRKTMDRAKY RELCRQYAEE QVQKQMTDFK RLGVMADWDN 150
PYITLQHEFE GQEIRVFGEM YKKGYIYKGK KPVYWSWSSE STLAEAEVEY 200
KDVEANSIFV AFPVVDSKGI IDPKDTYFVI WTTTPWTIPA NEAICVNPKF 250
DYSVVQVGDK KYVVATGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP 300
LYDKESLVTE GFHVTLDDGT GLVHTAPGFG ADDFNVGQKY DLPVFSPVDA 350
HGRYTDEVPE LEGMFYQDVD KLMVEKLKDA GALLKLKVFT HSYPHDWRTK 400
KPVIFRATTQ WFASIAPFRD QILEQIDNAK FIPSWGKTRL YNMIKDRGDW 450
VISRQRAWGV PLPIFYAEDG TPIVTPETIE HIAEIFDKEG SNAWYTHTAK 500
ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK RDGLHYPADL 550
YLEGSDQYRG WFNSSLITSV AVTGKAPYKE VLSQGFVLDD KGHKMSKSLG 600
NVISPNDVIK RMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT 650
FRYMLANTSD FDPKENGVAY DDLRSVDQYM EIKLNDLVAE CLAAYDKFDF 700
TTVFKKIFNF ISNDLSAFYL DFAKDVLYIE GKNSLERRSM QTVIYDAAVK 750
LTKILTPILP HTMEEIWGFL KEPEDYVQLA NMPKVENYTN HDELLENWGK 800
FMNLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM LDDLDADFRQ 850
ILIVSKLTIV DGEAPENAEK LNNASIVVEH AEGEVCPRCR MIRTDIGEDP 900
KLPELCERCA KIVEEDFPEA AQEGLEE 927
Length:927
Mass (Da):106,244
Last modified:March 1, 2005 - v1
Checksum:iF62F379E86F0D5E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000033 Genomic DNA. Translation: AAV42679.1.
RefSeqiYP_193710.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV42679; AAV42679; LBA0817.
GeneIDi3251646.
KEGGilac:LBA0817.
PATRICi22196728. VBILacAci7974_0779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000033 Genomic DNA. Translation: AAV42679.1 .
RefSeqi YP_193710.1. NC_006814.3.

3D structure databases

ProteinModelPortali Q5FKU5.
SMRi Q5FKU5. Positions 1-915.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272621.LBA0817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV42679 ; AAV42679 ; LBA0817 .
GeneIDi 3251646.
KEGGi lac:LBA0817.
PATRICi 22196728. VBILacAci7974_0779.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiSYI_LACAC
AccessioniPrimary (citable) accession number: Q5FKU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi