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Q5FKU5

- SYI_LACAC

UniProt

Q5FKU5 - SYI_LACAC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi886 – 8861ZincUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LBA0817
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligasePRO_0000098401Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272621.LBA0817.

Structurei

3D structure databases

ProteinModelPortaliQ5FKU5.
SMRiQ5FKU5. Positions 1-915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FKU5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIKDTLNLG KTKFKMRGNL PVREAEWEKE WEDNHLYEQR LKLNEGHPRF
60 70 80 90 100
DLHDGPPFAN GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP
110 120 130 140 150
VEQQLAKKGI DRKTMDRAKY RELCRQYAEE QVQKQMTDFK RLGVMADWDN
160 170 180 190 200
PYITLQHEFE GQEIRVFGEM YKKGYIYKGK KPVYWSWSSE STLAEAEVEY
210 220 230 240 250
KDVEANSIFV AFPVVDSKGI IDPKDTYFVI WTTTPWTIPA NEAICVNPKF
260 270 280 290 300
DYSVVQVGDK KYVVATGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP
310 320 330 340 350
LYDKESLVTE GFHVTLDDGT GLVHTAPGFG ADDFNVGQKY DLPVFSPVDA
360 370 380 390 400
HGRYTDEVPE LEGMFYQDVD KLMVEKLKDA GALLKLKVFT HSYPHDWRTK
410 420 430 440 450
KPVIFRATTQ WFASIAPFRD QILEQIDNAK FIPSWGKTRL YNMIKDRGDW
460 470 480 490 500
VISRQRAWGV PLPIFYAEDG TPIVTPETIE HIAEIFDKEG SNAWYTHTAK
510 520 530 540 550
ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK RDGLHYPADL
560 570 580 590 600
YLEGSDQYRG WFNSSLITSV AVTGKAPYKE VLSQGFVLDD KGHKMSKSLG
610 620 630 640 650
NVISPNDVIK RMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT
660 670 680 690 700
FRYMLANTSD FDPKENGVAY DDLRSVDQYM EIKLNDLVAE CLAAYDKFDF
710 720 730 740 750
TTVFKKIFNF ISNDLSAFYL DFAKDVLYIE GKNSLERRSM QTVIYDAAVK
760 770 780 790 800
LTKILTPILP HTMEEIWGFL KEPEDYVQLA NMPKVENYTN HDELLENWGK
810 820 830 840 850
FMNLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM LDDLDADFRQ
860 870 880 890 900
ILIVSKLTIV DGEAPENAEK LNNASIVVEH AEGEVCPRCR MIRTDIGEDP
910 920
KLPELCERCA KIVEEDFPEA AQEGLEE
Length:927
Mass (Da):106,244
Last modified:March 1, 2005 - v1
Checksum:iF62F379E86F0D5E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV42679.1.
RefSeqiYP_193710.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV42679; AAV42679; LBA0817.
GeneIDi3251646.
KEGGilac:LBA0817.
PATRICi22196728. VBILacAci7974_0779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV42679.1 .
RefSeqi YP_193710.1. NC_006814.3.

3D structure databases

ProteinModelPortali Q5FKU5.
SMRi Q5FKU5. Positions 1-915.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272621.LBA0817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV42679 ; AAV42679 ; LBA0817 .
GeneIDi 3251646.
KEGGi lac:LBA0817.
PATRICi 22196728. VBILacAci7974_0779.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiSYI_LACAC
AccessioniPrimary (citable) accession number: Q5FKU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3