Q5FKK7 (LDH2_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 2
Short name=L-LDH 2
EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	LBA0910

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	L-lactate dehydrogenase 2 HAMAP MF_00488		PRO_0000237548
Regions
	Nucleotide binding	11 – 39	29	NAD By similarity
Sites
	Active site	174	1	Proton acceptor
	Binding site	87	1	Substrate By similarity
	Binding site	119	1	NAD or substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FKK7 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: A9BC556A14F44E83	FASTA	308	33,349
	10 20 30 40 50 60 MSRKVFLVGD GAVGSTFAND LLQNTTVDEL AIFDVAKDRP VGDSMDLEDI TPFTGQTNIH 70 80 90 100 110 120 PAEYSDAKDA DVCVITAGVP RKPGETRLDL VNKNVKILKT IVDPVVESGF KGVFVVSANP 130 140 150 160 170 180 VDILTTLTQK ISGFPKDRVI GTGTSLDSMR LRVELAKKLN VPVAKVNSMV LGEHGDTSFE 190 200 210 220 230 240 NFDESTVDNK PLRDYSEIND NVLSEIESDV RKKGGKIITN KGATFYGVAM MLTQIVSAIL 250 260 270 280 290 300 DNRSICLPLS APINGEYGIK HDLYLGTPTI INGNGIEKVI ETKLSDVEKA KMINSADKMQ EVLSGVEM

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42767.1.
RefSeq	YP_193798.1. NC_006814.3.
3D structure databases
ProteinModelPortal	Q5FKK7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3251850.
GenomeReviews	Gene locus LBA0910 in contig CP000033_GR.
KEGG	lac:LBA0910.
NMPDR	fig|272621.3.peg.903.
PATRIC	22196908. VBILacAci7974_0868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	DYSEIND.
ProtClustDB	PRK00066.
Enzyme and pathway databases
BioCyc	LACI272621:LBA0910-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LACAC

Accession

Primary (citable) accession number: Q5FKK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program