Reviewed, UniProtKB/Swiss-Prot Q5FKK7 (LDH2_LACAC)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	LBA0910

Organism

Lactobacillus acidophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	L-lactate dehydrogenase 2		PRO_0000237548
Regions
	Nucleotide binding	11 – 39	29	NAD By similarity
Sites
	Active site	174	1	Proton acceptor
	Binding site	87	1	Substrate By similarity
	Binding site	119	1	NAD or substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FKK7-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: A9BC556A14F44E83	FASTA	308	33,349
	10 20 30 40 50 60 MSRKVFLVGD GAVGSTFAND LLQNTTVDEL AIFDVAKDRP VGDSMDLEDI TPFTGQTNIH 70 80 90 100 110 120 PAEYSDAKDA DVCVITAGVP RKPGETRLDL VNKNVKILKT IVDPVVESGF KGVFVVSANP 130 140 150 160 170 180 VDILTTLTQK ISGFPKDRVI GTGTSLDSMR LRVELAKKLN VPVAKVNSMV LGEHGDTSFE 190 200 210 220 230 240 NFDESTVDNK PLRDYSEIND NVLSEIESDV RKKGGKIITN KGATFYGVAM MLTQIVSAIL 250 260 270 280 290 300 DNRSICLPLS APINGEYGIK HDLYLGTPTI INGNGIEKVI ETKLSDVEKA KMINSADKMQ EVLSGVEM

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCFM.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000033 Genomic DNA. Translation: AAV42767.1.
RefSeq	YP_193798.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3251850.
GenomeReviews	Gene locus LBA0910 in contig CP000033_GR.
KEGG	lac:LBA0910.
NMPDR	fig|272621.3.peg.903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5FKK7.
Enzyme and pathway databases
BioCyc	LACI272621:LBA0910-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LACAC

Accession

Primary (citable) accession number: Q5FKK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2005
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)