Q5FKE4 (RNH2_LACAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	LBA0979

Organism

Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]

Taxonomic identifier

272621 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus acidophilus

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00052
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00052
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00052.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: HAMAP ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Ribonuclease HII HAMAP-Rule MF_00052		PRO_0000111581
Sites
	Metal binding	72	1	Divalent metal cation By similarity
	Metal binding	73	1	Divalent metal cation By similarity
	Metal binding	164	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FKE4 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: A9C82181F1968EA8	FASTA	250	28,092
	10 20 30 40 50 60 MTIKEIKELL KGDVSEEEIA NLEKDSRSGV QKLLISYRKK QKKLFEKKQA FLERFLYEKQ 70 80 90 100 110 120 FWQKGQLVAG VDEVGRGPLA GPVVTAAVII DQDFDLLDVN DSKKLSPEKR LKLYPKILEE 130 140 150 160 170 180 AVSVAVGVKN AQVIDQINIY EADRQAMAQA VNALDIKPDA LLVDAMNVPV DLPQIELIKG 190 200 210 220 230 240 DAKSNSIAAA SIVAKVFRDK LMDDYDKIYP HYGFSRNAGY GTKEHIDALK QYGPTHIHRK 250 TFAPVSDFFK

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42830.1.
RefSeq	YP_193861.1. NC_006814.3.
3D structure databases
ProteinModelPortal	Q5FKE4.
ModBase	Search...
Protein-protein interaction databases
STRING	272621.LBA0979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV42830; AAV42830; LBA0979.
GeneID	3251995.
KEGG	lac:LBA0979.
PATRIC	22197032. VBILacAci7974_0930.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HOG000100288.
KO	K03470.
OMA	NRCPKLA.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	LACI272621:GJO8-1601-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B.
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
PANTHER	PTHR10954. PTHR10954. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_LACAC

Accession

Primary (citable) accession number: Q5FKE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program