Reviewed, UniProtKB/Swiss-Prot Q5FJY2 (FTHS1_LACAC)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase 1
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase 1
      Short name=FHS 1
      Short name=FTHFS 1

Gene names

Name:	fhs1
Ordered Locus Names:	LBA1153

Organism

Lactobacillus acidophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Formate--tetrahydrofolate ligase 1 HAMAP MF_01543		PRO_0000199351
Regions
	Nucleotide binding	66 – 73	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FJY2-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: E5F4B694A0176CA1	FASTA	558	60,595
	10 20 30 40 50 60 MKSDIEIAQE TKELPIEEIA AKVNLKKEDL EPYGQDKAKI NWKAINRIRK NDKLGKLILV 70 80 90 100 110 120 TSISPTPAGE GKSTITIGLG DAIHNQLHKN TLIALREPSM GPVFGLKGGA TGGGRAQIIP 130 140 150 160 170 180 MEDINLHFTG DMHALTAAID TLAALVDNYI YQDNSLNIDP ERILLKRGLD VNDRALRKIT 190 200 210 220 230 240 VGQGSKFNGI EHKASFAITV ANELMAILCL ANDINDLKAR IGDMLVGYTQ DDQPVYVKQL 250 260 270 280 290 300 GFQGAIAALL SNALKPNLVQ TLEHTPALVH GGPFANIAHG ANSVMATNLA LHLSDYVLTE 310 320 330 340 350 360 AGFGSDLGGQ KFMDFVSKHL DKTPDAAVVV ATVRALKYQA LGSTDKLDEE NLDALKTGFK 370 380 390 400 410 420 NLERHMNNMR SYNVPVIVLI NRFDTDTDKE LELLKELVEK QGIKAEVVTY HNEGSKGGSR 430 440 450 460 470 480 AAQEVINLAD SGKAELISTY NEDDDIKSKI KKIATKIYHA DGVEYTDKAE EQIKELAKIG 490 500 510 520 530 540 KDKLPVIIAK TQYSFSDDKK KLGAPGGFNL HVKGVSLKNG ARFIVVTTGN VLDMPGLPKH 550 PAALDIDVDN DGKISGLF

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCFM/NCK56/N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV42992.1.
RefSeq	YP_194023.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3252922.
GenomeReviews	Gene locus LBA1153 in contig CP000033_GR.
KEGG	lac:LBA1153.
NMPDR	fig|272621.3.peg.1084.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	RALKYQA.
Enzyme and pathway databases
BioCyc	LACI272621:LBA1153-MONOMER.
BRENDA	6.3.4.3. 3045.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS1_LACAC

Accession

Primary (citable) accession number: Q5FJY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)