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Q5FJY2 (FTHS1_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase 1

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase 1
Short name=FHS 1
Short name=FTHFS 1
Gene names
Name:fhs1
Ordered Locus Names:LBA1153
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Formate--tetrahydrofolate ligase 1 HAMAP-Rule MF_01543
PRO_0000199351

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FJY2 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: E5F4B694A0176CA1

FASTA55860,595
        10         20         30         40         50         60 
MKSDIEIAQE TKELPIEEIA AKVNLKKEDL EPYGQDKAKI NWKAINRIRK NDKLGKLILV 

        70         80         90        100        110        120 
TSISPTPAGE GKSTITIGLG DAIHNQLHKN TLIALREPSM GPVFGLKGGA TGGGRAQIIP 

       130        140        150        160        170        180 
MEDINLHFTG DMHALTAAID TLAALVDNYI YQDNSLNIDP ERILLKRGLD VNDRALRKIT 

       190        200        210        220        230        240 
VGQGSKFNGI EHKASFAITV ANELMAILCL ANDINDLKAR IGDMLVGYTQ DDQPVYVKQL 

       250        260        270        280        290        300 
GFQGAIAALL SNALKPNLVQ TLEHTPALVH GGPFANIAHG ANSVMATNLA LHLSDYVLTE 

       310        320        330        340        350        360 
AGFGSDLGGQ KFMDFVSKHL DKTPDAAVVV ATVRALKYQA LGSTDKLDEE NLDALKTGFK 

       370        380        390        400        410        420 
NLERHMNNMR SYNVPVIVLI NRFDTDTDKE LELLKELVEK QGIKAEVVTY HNEGSKGGSR 

       430        440        450        460        470        480 
AAQEVINLAD SGKAELISTY NEDDDIKSKI KKIATKIYHA DGVEYTDKAE EQIKELAKIG 

       490        500        510        520        530        540 
KDKLPVIIAK TQYSFSDDKK KLGAPGGFNL HVKGVSLKNG ARFIVVTTGN VLDMPGLPKH 

       550 
PAALDIDVDN DGKISGLF 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV42992.1.
RefSeqYP_194023.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FJY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA1153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV42992; AAV42992; LBA1153.
GeneID3252922.
KEGGlac:LBA1153.
PATRIC22197360. VBILacAci7974_1094.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMAARFIVVT.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS1_LACAC
AccessionPrimary (citable) accession number: Q5FJY2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways