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Q5FJB3 (PYRF_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orotidine 5'-phosphate decarboxylase

EC=4.1.1.23
Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase
Gene names
Name:pyrF
Ordered Locus Names:LBA1386
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP-Rule MF_01200

Catalytic activity

Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01200

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP-Rule MF_01200

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01200

Sequence similarities

Belongs to the OMP decarboxylase family. Type 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 235235Orotidine 5'-phosphate decarboxylase HAMAP-Rule MF_01200
PRO_0000241868

Regions

Region60 – 6910Substrate binding By similarity

Sites

Active site621Proton donor By similarity
Binding site101Substrate By similarity
Binding site331Substrate By similarity
Binding site1231Substrate By similarity
Binding site1851Substrate By similarity
Binding site1941Substrate By similarity
Binding site2141Substrate; via amide nitrogen By similarity
Binding site2151Substrate By similarity

Secondary structure

............................................... 235
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5FJB3 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 892076F9C45D7B1F

FASTA23525,404
        10         20         30         40         50         60 
MDRPVIVALD LDNEEQLNKI LSKLGDPHDV FVKVGMELFY NAGIDVIKKL TQQGYKIFLD 

        70         80         90        100        110        120 
LKMHDIPNTV YNGAKALAKL GITFTTVHAL GGSQMIKSAK DGLIAGTPAG HSVPKLLAVT 

       130        140        150        160        170        180 
ELTSISDDVL RNEQNCRLPM AEQVLSLAKM AKHSGADGVI CSPLEVKKLH ENIGDDFLYV 

       190        200        210        220        230 
TPGIRPAGNA KDDQSRVATP KMAKEWGSSA IVVGRPITLA SDPKAAYEAI KKEFN 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV43211.1.
RefSeqYP_194242.1. NC_006814.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TFXX-ray2.19A/B1-235[»]
ProteinModelPortalQ5FJB3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA1386.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV43211; AAV43211; LBA1386.
GeneID3252592.
KEGGlac:LBA1386.
PATRIC22197796. VBILacAci7974_1311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0284.
HOGENOMHOG000226071.
KOK01591.
OMARPITQSA.
OrthoDBEOG6N6815.

Enzyme and pathway databases

UniPathwayUPA00070; UER00120.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01200_B. OMPdecase_type1_B.
InterProIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
TIGRFAMsTIGR01740. pyrF. 1 hit.
PROSITEPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRF_LACAC
AccessionPrimary (citable) accession number: Q5FJB3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways