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Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=3.84 mM for o-nitrophenyl-beta-D-galactopyranoside (ONPG) (at 37 degrees Celsius and pH 6.0)1 Publication
  2. KM=88.9 mM for lactose (at 37 degrees Celsius and pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 6.0 for both lactose and ONPG hydrolysis, but lactose hydrolysis activity is more sensitive to changes in pH than ONPG hydrolysis activity.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius for both lactose and ONPG hydrolysis. Retains 16.5% of activity for ONPG hydrolysis and 11.2% of activity for lactose hydrolysis at 4 degrees Celsius. Stable at or below 25 degrees Celsius, but loses 50% of its activity after 1 hour at 37 degrees Celsius and is inactivated after only 40 minutes at 45 degrees Celsius. Can hydrolyze 73% of lactose in milk in 30 hours at 10 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei109SubstrateBy similarity1
    Metal bindingi113ZincBy similarity1
    Binding sitei147SubstrateBy similarity1
    Active sitei148Proton donorBy similarity1
    Metal bindingi153ZincBy similarity1
    Metal bindingi155ZincBy similarity1
    Metal bindingi158ZincBy similarity1
    Active sitei307NucleophileBy similarity1
    Binding sitei315SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciLACI272621:G1G49-1432-MONOMER

    Protein family/group databases

    CAZyiGH42 Glycoside Hydrolase Family 42

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase LacZ (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:lacZImported
    Ordered Locus Names:LBA1462
    OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
    Taxonomic identifieri272621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    Proteomesi
    • UP000006381 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Biotechnological usei

    Has potential for lactose removal from dairy products at refrigerated temperatures.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004076901 – 667Beta-galactosidase LacZAdd BLAST667

    Proteomic databases

    PRIDEiQ5FJ41

    Interactioni

    Protein-protein interaction databases

    STRINGi272621.LBA1462

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FJ41
    SMRiQ5FJ41
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni355 – 358Substrate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence analysis

    Phylogenomic databases

    eggNOGiENOG4105C4C Bacteria
    COG1874 LUCA
    HOGENOMiHOG000117811
    KOiK12308
    OMAiSRRHYCF

    Family and domain databases

    Gene3Di2.60.40.1180, 1 hit
    3.40.50.880, 1 hit
    InterProiView protein in InterPro
    IPR013739 Beta_galactosidase_C
    IPR013738 Beta_galactosidase_Trimer
    IPR029062 Class_I_gatase-like
    IPR003476 Glyco_hydro_42
    IPR013529 Glyco_hydro_42_N
    IPR013780 Glyco_hydro_b
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR36447 PTHR36447, 1 hit
    PfamiView protein in Pfam
    PF02449 Glyco_hydro_42, 1 hit
    PF08533 Glyco_hydro_42C, 1 hit
    PF08532 Glyco_hydro_42M, 1 hit
    PIRSFiPIRSF001084 B-galactosidase, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit
    SSF52317 SSF52317, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q5FJ41-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP
    60 70 80 90 100
    REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ
    110 120 130 140 150
    DRRHVFGQRH NFCPNSSNYQ RLAGELVKQL VERYKDNKHI VVWHINNEYG
    160 170 180 190 200
    GNCYCENCQN AFRKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE
    210 220 230 240 250
    LGDVWGIEGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKYDPETPVT
    260 270 280 290 300
    TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH
    310 320 330 340 350
    QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFQLKQA
    360 370 380 390 400
    VGGSEKFHSA VIAHSQRTDT RVFKELADLG KKLKNAGPTI LGSKTKAKVA
    410 420 430 440 450
    IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF
    460 470 480 490 500
    SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG
    510 520 530 540 550
    GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK
    560 570 580 590 600
    ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG
    610 620 630 640 650
    VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG
    660
    EKAHKDMKGW DVQVLRN
    Length:667
    Mass (Da):76,584
    Last modified:March 1, 2005 - v1
    Checksum:i0034A0EC1C4438D2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU590652 Genomic DNA Translation: ACC38288.1
    CP000033 Genomic DNA Translation: AAV43283.1
    RefSeqiWP_011254470.1, NC_006814.3
    YP_194314.1, NC_006814.3

    Genome annotation databases

    EnsemblBacteriaiAAV43283; AAV43283; LBA1462
    GeneIDi3251504
    KEGGilac:LBA1462
    PATRICifig|272621.13.peg.1384

    Similar proteinsi

    Entry informationi

    Entry nameiBGAL1_LACAC
    AccessioniPrimary (citable) accession number: Q5FJ41
    Secondary accession number(s): B2LWG4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 1, 2005
    Last modified: May 23, 2018
    This is version 68 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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