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Q5FJ41

- BGAL1_LACAC

UniProt

Q5FJ41 - BGAL1_LACAC

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Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=3.84 mM for o-nitrophenyl-beta-D-galactopyranoside (ONPG) (at 37 degrees Celsius and pH 6.0)1 Publication
  2. KM=88.9 mM for lactose (at 37 degrees Celsius and pH 6.0)1 Publication

pH dependencei

Optimum pH is 6.0 for both lactose and ONPG hydrolysis, but lactose hydrolysis activity is more sensitive to changes in pH than ONPG hydrolysis activity.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius for both lactose and ONPG hydrolysis. Retains 16.5% of activity for ONPG hydrolysis and 11.2% of activity for lactose hydrolysis at 4 degrees Celsius. Stable at or below 25 degrees Celsius, but loses 50% of its activity after 1 hour at 37 degrees Celsius and is inactivated after only 40 minutes at 45 degrees Celsius. Can hydrolyze 73% of lactose in milk in 30 hours at 10 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateBy similarity
Metal bindingi113 – 1131ZincBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Active sitei148 – 1481Proton donorBy similarity
Metal bindingi153 – 1531ZincBy similarity
Metal bindingi155 – 1551ZincBy similarity
Metal bindingi158 – 1581ZincBy similarity
Active sitei307 – 3071NucleophileBy similarity
Binding sitei315 – 3151SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacZ (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:lacZImported
Ordered Locus Names:LBA1462
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

Has potential for lactose removal from dairy products at refrigerated temperatures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Beta-galactosidase LacZPRO_0000407690Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272621.LBA1462.

Structurei

3D structure databases

ProteinModelPortaliQ5FJ41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 3584Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000117811.
KOiK12308.
OMAiLFDWENW.
OrthoDBiEOG6GTZGG.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5FJ41 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP
60 70 80 90 100
REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ
110 120 130 140 150
DRRHVFGQRH NFCPNSSNYQ RLAGELVKQL VERYKDNKHI VVWHINNEYG
160 170 180 190 200
GNCYCENCQN AFRKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE
210 220 230 240 250
LGDVWGIEGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKYDPETPVT
260 270 280 290 300
TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH
310 320 330 340 350
QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFQLKQA
360 370 380 390 400
VGGSEKFHSA VIAHSQRTDT RVFKELADLG KKLKNAGPTI LGSKTKAKVA
410 420 430 440 450
IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF
460 470 480 490 500
SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG
510 520 530 540 550
GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK
560 570 580 590 600
ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG
610 620 630 640 650
VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG
660
EKAHKDMKGW DVQVLRN
Length:667
Mass (Da):76,584
Last modified:March 1, 2005 - v1
Checksum:i0034A0EC1C4438D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU590652 Genomic DNA. Translation: ACC38288.1.
CP000033 Genomic DNA. Translation: AAV43283.1.
RefSeqiWP_011254470.1. NC_006814.3.
YP_194314.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV43283; AAV43283; LBA1462.
GeneIDi3251504.
KEGGilac:LBA1462.
PATRICi22197942. VBILacAci7974_1384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU590652 Genomic DNA. Translation: ACC38288.1 .
CP000033 Genomic DNA. Translation: AAV43283.1 .
RefSeqi WP_011254470.1. NC_006814.3.
YP_194314.1. NC_006814.3.

3D structure databases

ProteinModelPortali Q5FJ41.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272621.LBA1462.

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV43283 ; AAV43283 ; LBA1462 .
GeneIDi 3251504.
KEGGi lac:LBA1462.
PATRICi 22197942. VBILacAci7974_1384.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000117811.
KOi K12308.
OMAi LFDWENW.
OrthoDBi EOG6GTZGG.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional identification of a putative beta-galactosidase gene in the special lac gene cluster of Lactobacillus acidophilus."
    Pan Q., Zhu J., Liu L., Cong Y., Hu F., Li J., Yu X.
    Curr. Microbiol. 60:172-178(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY.
    Strain: ATCC 4356 / DSM 20079 / NBRC 13951 / JCM 1132 / NCIMB 8690.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiBGAL1_LACAC
AccessioniPrimary (citable) accession number: Q5FJ41
Secondary accession number(s): B2LWG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 1, 2005
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3