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Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=3.84 mM for o-nitrophenyl-beta-D-galactopyranoside (ONPG) (at 37 degrees Celsius and pH 6.0)1 Publication
  2. KM=88.9 mM for lactose (at 37 degrees Celsius and pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 6.0 for both lactose and ONPG hydrolysis, but lactose hydrolysis activity is more sensitive to changes in pH than ONPG hydrolysis activity.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius for both lactose and ONPG hydrolysis. Retains 16.5% of activity for ONPG hydrolysis and 11.2% of activity for lactose hydrolysis at 4 degrees Celsius. Stable at or below 25 degrees Celsius, but loses 50% of its activity after 1 hour at 37 degrees Celsius and is inactivated after only 40 minutes at 45 degrees Celsius. Can hydrolyze 73% of lactose in milk in 30 hours at 10 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateBy similarity
    Metal bindingi113 – 1131ZincBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei148 – 1481Proton donorBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi155 – 1551ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Active sitei307 – 3071NucleophileBy similarity
    Binding sitei315 – 3151SubstrateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase LacZ (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:lacZImported
    Ordered Locus Names:LBA1462
    OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
    Taxonomic identifieri272621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000006381 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Biotechnological usei

    Has potential for lactose removal from dairy products at refrigerated temperatures.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 667667Beta-galactosidase LacZPRO_0000407690Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272621.LBA1462.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FJ41.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 3584Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiDWENWWA.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5FJ41-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP
    60 70 80 90 100
    REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ
    110 120 130 140 150
    DRRHVFGQRH NFCPNSSNYQ RLAGELVKQL VERYKDNKHI VVWHINNEYG
    160 170 180 190 200
    GNCYCENCQN AFRKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE
    210 220 230 240 250
    LGDVWGIEGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKYDPETPVT
    260 270 280 290 300
    TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH
    310 320 330 340 350
    QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFQLKQA
    360 370 380 390 400
    VGGSEKFHSA VIAHSQRTDT RVFKELADLG KKLKNAGPTI LGSKTKAKVA
    410 420 430 440 450
    IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF
    460 470 480 490 500
    SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG
    510 520 530 540 550
    GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK
    560 570 580 590 600
    ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG
    610 620 630 640 650
    VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG
    660
    EKAHKDMKGW DVQVLRN
    Length:667
    Mass (Da):76,584
    Last modified:March 1, 2005 - v1
    Checksum:i0034A0EC1C4438D2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU590652 Genomic DNA. Translation: ACC38288.1.
    CP000033 Genomic DNA. Translation: AAV43283.1.
    RefSeqiWP_011254470.1. NC_006814.3.
    YP_194314.1. NC_006814.3.

    Genome annotation databases

    EnsemblBacteriaiAAV43283; AAV43283; LBA1462.
    GeneIDi3251504.
    KEGGilac:LBA1462.
    PATRICi22197942. VBILacAci7974_1384.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU590652 Genomic DNA. Translation: ACC38288.1.
    CP000033 Genomic DNA. Translation: AAV43283.1.
    RefSeqiWP_011254470.1. NC_006814.3.
    YP_194314.1. NC_006814.3.

    3D structure databases

    ProteinModelPortaliQ5FJ41.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272621.LBA1462.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAV43283; AAV43283; LBA1462.
    GeneIDi3251504.
    KEGGilac:LBA1462.
    PATRICi22197942. VBILacAci7974_1384.

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiDWENWWA.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Functional identification of a putative beta-galactosidase gene in the special lac gene cluster of Lactobacillus acidophilus."
      Pan Q., Zhu J., Liu L., Cong Y., Hu F., Li J., Yu X.
      Curr. Microbiol. 60:172-178(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY.
      Strain: ATCC 4356 / DSM 20079 / NBRC 13951 / JCM 1132 / NCIMB 8690.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700396 / NCK56 / N2 / NCFM.

    Entry informationi

    Entry nameiBGAL1_LACAC
    AccessioniPrimary (citable) accession number: Q5FJ41
    Secondary accession number(s): B2LWG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 1, 2005
    Last modified: January 7, 2015
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.