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Q5FJ41

- BGAL1_LACAC

UniProt

Q5FJ41 - BGAL1_LACAC

Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Kineticsi

    1. KM=3.84 mM for o-nitrophenyl-beta-D-galactopyranoside (ONPG) (at 37 degrees Celsius and pH 6.0)1 Publication
    2. KM=88.9 mM for lactose (at 37 degrees Celsius and pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 6.0 for both lactose and ONPG hydrolysis, but lactose hydrolysis activity is more sensitive to changes in pH than ONPG hydrolysis activity.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius for both lactose and ONPG hydrolysis. Retains 16.5% of activity for ONPG hydrolysis and 11.2% of activity for lactose hydrolysis at 4 degrees Celsius. Stable at or below 25 degrees Celsius, but loses 50% of its activity after 1 hour at 37 degrees Celsius and is inactivated after only 40 minutes at 45 degrees Celsius. Can hydrolyze 73% of lactose in milk in 30 hours at 10 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateBy similarity
    Metal bindingi113 – 1131ZincBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei148 – 1481Proton donorBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi155 – 1551ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Active sitei307 – 3071NucleophileBy similarity
    Binding sitei315 – 3151SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase LacZ (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:lacZImported
    Ordered Locus Names:LBA1462
    OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
    Taxonomic identifieri272621 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000006381: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    Pathology & Biotechi

    Biotechnological usei

    Has potential for lactose removal from dairy products at refrigerated temperatures.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 667667Beta-galactosidase LacZPRO_0000407690Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272621.LBA1462.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FJ41.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 3584Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiLFDWENW.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5FJ41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP    50
    REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ 100
    DRRHVFGQRH NFCPNSSNYQ RLAGELVKQL VERYKDNKHI VVWHINNEYG 150
    GNCYCENCQN AFRKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE 200
    LGDVWGIEGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKYDPETPVT 250
    TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH 300
    QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFQLKQA 350
    VGGSEKFHSA VIAHSQRTDT RVFKELADLG KKLKNAGPTI LGSKTKAKVA 400
    IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF 450
    SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG 500
    GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK 550
    ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG 600
    VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG 650
    EKAHKDMKGW DVQVLRN 667
    Length:667
    Mass (Da):76,584
    Last modified:March 1, 2005 - v1
    Checksum:i0034A0EC1C4438D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU590652 Genomic DNA. Translation: ACC38288.1.
    CP000033 Genomic DNA. Translation: AAV43283.1.
    RefSeqiWP_011254470.1. NC_006814.3.
    YP_194314.1. NC_006814.3.

    Genome annotation databases

    EnsemblBacteriaiAAV43283; AAV43283; LBA1462.
    GeneIDi3251504.
    KEGGilac:LBA1462.
    PATRICi22197942. VBILacAci7974_1384.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU590652 Genomic DNA. Translation: ACC38288.1 .
    CP000033 Genomic DNA. Translation: AAV43283.1 .
    RefSeqi WP_011254470.1. NC_006814.3.
    YP_194314.1. NC_006814.3.

    3D structure databases

    ProteinModelPortali Q5FJ41.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272621.LBA1462.

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV43283 ; AAV43283 ; LBA1462 .
    GeneIDi 3251504.
    KEGGi lac:LBA1462.
    PATRICi 22197942. VBILacAci7974_1384.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000117811.
    KOi K12308.
    OMAi LFDWENW.
    OrthoDBi EOG6GTZGG.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional identification of a putative beta-galactosidase gene in the special lac gene cluster of Lactobacillus acidophilus."
      Pan Q., Zhu J., Liu L., Cong Y., Hu F., Li J., Yu X.
      Curr. Microbiol. 60:172-178(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY.
      Strain: ATCC 4356 / DSM 20079 / NBRC 13951 / JCM 1132 / NCIMB 8690.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700396 / NCK56 / N2 / NCFM.

    Entry informationi

    Entry nameiBGAL1_LACAC
    AccessioniPrimary (citable) accession number: Q5FJ41
    Secondary accession number(s): B2LWG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3