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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

BioCyciLACI272621:G1G49-1517-MONOMER
UniPathwayiUPA00074; UER00133
UPA00074; UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:LBA1552
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000006381 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000188961 – 513Bifunctional purine biosynthesis protein PurHAdd BLAST513

Proteomic databases

PRIDEiQ5FIV5

Interactioni

Protein-protein interaction databases

STRINGi272621.LBA1552

Structurei

3D structure databases

ProteinModelPortaliQ5FIV5
SMRiQ5FIV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 144MGS-likePROSITE-ProRule annotationAdd BLAST144

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DC1 Bacteria
COG0138 LUCA
HOGENOMiHOG000230373
KOiK00602
OMAiDLLFAWK

Family and domain databases

Gene3Di3.40.140.20, 2 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q5FIV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRALISVSD KTNLVDFAKG LVQNGYEIIS TGGTKKTLDE AGVKTISVEE
60 70 80 90 100
VTNFPEILDG RVKTLNPYIH GGLLAKRDDL EHMATLEKLN IKPIDLVCVN
110 120 130 140 150
LYPFKQTIEK PDVTTEEAIE NIDIGGPSLL RAASKNYQDV TVVTDKNDYD
160 170 180 190 200
LVLKEIAEKG NTTLETRAKL AAKVFRATAA YDAVIANYLT KQVGLEDPEK
210 220 230 240 250
LTLTYDLKEK MRYGENSHQK AWLYEDAIPK SFSILQAHQL HGKKLSYNNI
260 270 280 290 300
KDADEALRCI REFQDEPTVV AMKHMNPCGI GRGDTLEEAW DRAYEADSVS
310 320 330 340 350
IFGGVIALNR KVDLATAKKM HKIFLEIIIA PGFDDDALEV LEKKKNIRLL
360 370 380 390 400
KLDFSKENEP TRPEIVSVMG GILQQEQDTL VENTDDWKVV TNAQPTEAQL
410 420 430 440 450
KTMMFALKAV KHTKSNAIVV ANDERTLGVG AGQPNRIDSA KIAIKHAGDA
460 470 480 490 500
IDDRAVLSSD AFFPFNDCVE YAAKHGIKAI VQPGGSIRDK DSIEIADKYG
510
VAMVFTGYRH FRH
Length:513
Mass (Da):57,008
Last modified:March 1, 2005 - v1
Checksum:i9BED709279B278C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA Translation: AAV43369.1
RefSeqiWP_003548356.1, NC_006814.3
YP_194400.1, NC_006814.3

Genome annotation databases

EnsemblBacteriaiAAV43369; AAV43369; LBA1552
GeneIDi3251379
KEGGilac:LBA1552
PATRICifig|272621.13.peg.1474

Similar proteinsi

Entry informationi

Entry nameiPUR9_LACAC
AccessioniPrimary (citable) accession number: Q5FIV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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