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Protein

Formate--tetrahydrofolate ligase 2

Gene

fhs2

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 708ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase 2UniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetase 2UniRule annotation
Short name:
FHS 2UniRule annotation
Short name:
FTHFS 2UniRule annotation
Gene namesi
Name:fhs2UniRule annotation
Ordered Locus Names:LBA1562
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Formate--tetrahydrofolate ligase 2PRO_0000199352Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272621.LBA1562.

Structurei

3D structure databases

ProteinModelPortaliQ5FIU5.
SMRiQ5FIU5. Positions 4-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FIU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTDIEIADA AKMQPITEIA QKLGLSSDDI EQYGHYKAKI NLPVKEVEGK
60 70 80 90 100
KHKLILVTGI NPTSAGEGKS TVLVGLGDAL NQLGHQTTIA MREPSMGPVF
110 120 130 140 150
GIKGGATGGG YSQVVPMEDI NLHFTGDMHA LTSANNTLAA LIDNYIMRDN
160 170 180 190 200
VLGLDPRRII WKRVEDVNDR VLRNVVTGLG GIMQGVPRET GFDITAASEM
210 220 230 240 250
MAILCLSESL HNLKKRIGRI VVGYTYEKKP VTVDQLGFTG AITLLLKDAI
260 270 280 290 300
KPNLVQTLDH TPAIIHGGPF ANIAHGCNSV LATQIALKTS DYTVTECGFG
310 320 330 340 350
ADLGAEKFLD IKRPVLGETP NAVVIVATAR ALKLNGGADK DELTKEDIPA
360 370 380 390 400
LEKGMANLRR HVENMQKYNL PIVVAINHFN SDTEAEIKTI QDACAEMGVK
410 420 430 440 450
AIEVDAWAKG GQGTQDLAKE VVKLADEELE FHELYKNSDS IEDKLSAIAT
460 470 480 490 500
KIYGAKRVVL SKKAKRQIKT FNEQGWNDLP VCIAKTQYSF TDDQTKLGAP
510 520 530 540 550
TDFDFHIRDL VPKLGAGFIV ALSGTMMTMP GLAKHPAAEN MDIDDNGQIA

GLF
Length:553
Mass (Da):59,847
Last modified:March 1, 2005 - v1
Checksum:i09FF57B54CB4EEF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV43379.1.
RefSeqiWP_003548380.1. NC_006814.3.
YP_194410.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV43379; AAV43379; LBA1562.
GeneIDi3251186.
KEGGilac:LBA1562.
PATRICi22198142. VBILacAci7974_1484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV43379.1.
RefSeqiWP_003548380.1. NC_006814.3.
YP_194410.1. NC_006814.3.

3D structure databases

ProteinModelPortaliQ5FIU5.
SMRiQ5FIU5. Positions 4-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272621.LBA1562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV43379; AAV43379; LBA1562.
GeneIDi3251186.
KEGGilac:LBA1562.
PATRICi22198142. VBILacAci7974_1484.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiFTHS2_LACAC
AccessioniPrimary (citable) accession number: Q5FIU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2005
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.