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Q5FIU5 (FTHS2_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase 2

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase 2
Short name=FHS 2
Short name=FTHFS 2
Gene names
Name:fhs2
Ordered Locus Names:LBA1562
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Formate--tetrahydrofolate ligase 2 HAMAP-Rule MF_01543
PRO_0000199352

Regions

Nucleotide binding63 – 708ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FIU5 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 09FF57B54CB4EEF8

FASTA55359,847
        10         20         30         40         50         60 
MPTDIEIADA AKMQPITEIA QKLGLSSDDI EQYGHYKAKI NLPVKEVEGK KHKLILVTGI 

        70         80         90        100        110        120 
NPTSAGEGKS TVLVGLGDAL NQLGHQTTIA MREPSMGPVF GIKGGATGGG YSQVVPMEDI 

       130        140        150        160        170        180 
NLHFTGDMHA LTSANNTLAA LIDNYIMRDN VLGLDPRRII WKRVEDVNDR VLRNVVTGLG 

       190        200        210        220        230        240 
GIMQGVPRET GFDITAASEM MAILCLSESL HNLKKRIGRI VVGYTYEKKP VTVDQLGFTG 

       250        260        270        280        290        300 
AITLLLKDAI KPNLVQTLDH TPAIIHGGPF ANIAHGCNSV LATQIALKTS DYTVTECGFG 

       310        320        330        340        350        360 
ADLGAEKFLD IKRPVLGETP NAVVIVATAR ALKLNGGADK DELTKEDIPA LEKGMANLRR 

       370        380        390        400        410        420 
HVENMQKYNL PIVVAINHFN SDTEAEIKTI QDACAEMGVK AIEVDAWAKG GQGTQDLAKE 

       430        440        450        460        470        480 
VVKLADEELE FHELYKNSDS IEDKLSAIAT KIYGAKRVVL SKKAKRQIKT FNEQGWNDLP 

       490        500        510        520        530        540 
VCIAKTQYSF TDDQTKLGAP TDFDFHIRDL VPKLGAGFIV ALSGTMMTMP GLAKHPAAEN 

       550 
MDIDDNGQIA GLF 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV43379.1.
RefSeqYP_194410.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FIU5.
SMRQ5FIU5. Positions 4-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA1562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV43379; AAV43379; LBA1562.
GeneID3251186.
KEGGlac:LBA1562.
PATRIC22198142. VBILacAci7974_1484.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMALKHHGGV.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS2_LACAC
AccessionPrimary (citable) accession number: Q5FIU5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways