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Q5FIS5 (TRMB_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:LBA1582
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229168

Regions

Region125 – 1306Interaction with RNA Potential
Region195 – 1984Substrate binding Potential

Sites

Binding site451S-adenosyl-L-methionine By similarity
Binding site701S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1231Substrate By similarity
Binding site1551Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q5FIS5 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 4B90FC0B0C35659D

FASTA21825,360
        10         20         30         40         50         60 
MRLRNKPWAV KLVNDHPESV LQNPNPDEKI YWEKRFGNDH PIEIEVGSGK GHFITTLAEQ 

        70         80         90        100        110        120 
HPEKNFVALE LQTTAAGIIL RTKLKKGLDN LQILRGDAAD INCFFDKNTT DVIYLNFSDP 

       130        140        150        160        170        180 
WPKSRHEKRR LTYKSFLNKY QQVLKPEGHI EFKTDNSGLF AYSVQSMNNY GMFFDFVSVD 

       190        200        210 
LHHEKPEIVE KNIETEYEHK FAAKGNPIYA LHAHFETK

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000033 Genomic DNA. Translation: AAV43399.1.
RefSeqYP_194430.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FIS5.
ModBaseSearch...

Protein-protein interaction databases

STRING272621.LBA1582.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV43399; AAV43399; LBA1582.
GeneID3251544.
KEGGlac:LBA1582.
PATRIC22198183. VBILacAci7974_1504.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMARLRNKPW.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycLACI272621:GJO8-2559-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_LACAC
AccessionPrimary (citable) accession number: Q5FIS5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: March 1, 2005
Last modified: May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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