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Q5FIP3 (SYL_LACAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LBA1617
OrganismLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) [Complete proteome] [HAMAP]
Taxonomic identifier272621 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091324

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif573 – 5775"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FIP3 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 0140B1FBDD5040B2

FASTA80492,469
        10         20         30         40         50         60 
MYNHKVVEKK WQDYWAKHDT FKTGTDSNKK NYYALDMFPF PSGKGLHVGH PEGYTATDIV 

        70         80         90        100        110        120 
SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEVVTK NNIANFKRQL NKLGFSYDWD 

       130        140        150        160        170        180 
REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA EVPVNWSPDL GTVVANEEIV DGKTERGGYP 

       190        200        210        220        230        240 
VYRRNMRQWM LKMTAYADRL LEDLDDLDWP EPVKEMQRNW IGRSLGAQVT FKIKDSDKTF 

       250        260        270        280        290        300 
DIFTTRPDTL FGCSYTVLAP ENKLVQEITT DAQRDEVNAY IKKIESKSDL ERTDLNKDKT 

       310        320        330        340        350        360 
GVFTGAYAIN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DERDYAFATK FGLPINPVLE 

       370        380        390        400        410        420 
GGDITKEAFT EDGPHINSEF LNGLNIKDAK KKMVEWLEEH NCGEKKVNYK LRDWDFSRQR 

       430        440        450        460        470        480 
YWGEPIPVIH WEDGETTLVP EDQLPLRLPH ATDIKPSGTP ESPLANLTDW VNVVDENGRK 

       490        500        510        520        530        540 
GKRETNTMPN WAGSSWYYLR YVDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDDVIDE YGADSLRMYE 

       610        620        630        640        650        660 
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDEND GELDKVYAET 

       670        680        690        700        710        720 
VKKVTEDFDA LHFNTAISQM MVFMNAAQKA KTIPREYAEG FVKLLAPVAP HMMEEIWQVF 

       730        740        750        760        770        780 
GHDESISYAE WSTYDPAKLV ESTVEIMVQV NGKLRGKFQA AKDADRDEVQ KQAMELPHVQ 

       790        800 
KFLEGKDVKK VIVVPNKIVN IVAK 

« Hide

References

[1]"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM."
Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R.
Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000033 Genomic DNA. Translation: AAV43431.1.
RefSeqYP_194462.1. NC_006814.3.

3D structure databases

ProteinModelPortalQ5FIP3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272621.LBA1617.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV43431; AAV43431; LBA1617.
GeneID3251380.
KEGGlac:LBA1617.
PATRIC22198251. VBILacAci7974_1537.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACAC
AccessionPrimary (citable) accession number: Q5FIP3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries