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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LBA1617
OrganismiLactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Taxonomic identifieri272621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000006381 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_1000091324Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272621.LBA1617.

Structurei

3D structure databases

ProteinModelPortaliQ5FIP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi573 – 5775"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FIP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHKVVEKK WQDYWAKHDT FKTGTDSNKK NYYALDMFPF PSGKGLHVGH
60 70 80 90 100
PEGYTATDIV SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEVVTK
110 120 130 140 150
NNIANFKRQL NKLGFSYDWD REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA
160 170 180 190 200
EVPVNWSPDL GTVVANEEIV DGKTERGGYP VYRRNMRQWM LKMTAYADRL
210 220 230 240 250
LEDLDDLDWP EPVKEMQRNW IGRSLGAQVT FKIKDSDKTF DIFTTRPDTL
260 270 280 290 300
FGCSYTVLAP ENKLVQEITT DAQRDEVNAY IKKIESKSDL ERTDLNKDKT
310 320 330 340 350
GVFTGAYAIN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DERDYAFATK
360 370 380 390 400
FGLPINPVLE GGDITKEAFT EDGPHINSEF LNGLNIKDAK KKMVEWLEEH
410 420 430 440 450
NCGEKKVNYK LRDWDFSRQR YWGEPIPVIH WEDGETTLVP EDQLPLRLPH
460 470 480 490 500
ATDIKPSGTP ESPLANLTDW VNVVDENGRK GKRETNTMPN WAGSSWYYLR
510 520 530 540 550
YVDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR FWHKVLYDLG
560 570 580 590 600
VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDDVIDE YGADSLRMYE
610 620 630 640 650
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDEND
660 670 680 690 700
GELDKVYAET VKKVTEDFDA LHFNTAISQM MVFMNAAQKA KTIPREYAEG
710 720 730 740 750
FVKLLAPVAP HMMEEIWQVF GHDESISYAE WSTYDPAKLV ESTVEIMVQV
760 770 780 790 800
NGKLRGKFQA AKDADRDEVQ KQAMELPHVQ KFLEGKDVKK VIVVPNKIVN

IVAK
Length:804
Mass (Da):92,469
Last modified:March 1, 2005 - v1
Checksum:i0140B1FBDD5040B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV43431.1.
RefSeqiWP_003548508.1. NC_006814.3.
YP_194462.1. NC_006814.3.

Genome annotation databases

EnsemblBacteriaiAAV43431; AAV43431; LBA1617.
GeneIDi3251380.
KEGGilac:LBA1617.
PATRICi22198251. VBILacAci7974_1537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000033 Genomic DNA. Translation: AAV43431.1.
RefSeqiWP_003548508.1. NC_006814.3.
YP_194462.1. NC_006814.3.

3D structure databases

ProteinModelPortaliQ5FIP3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272621.LBA1617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV43431; AAV43431; LBA1617.
GeneIDi3251380.
KEGGilac:LBA1617.
PATRICi22198251. VBILacAci7974_1537.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700396 / NCK56 / N2 / NCFM.

Entry informationi

Entry nameiSYL_LACAC
AccessioniPrimary (citable) accession number: Q5FIP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2005
Last modified: May 27, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.