Reviewed, UniProtKB/Swiss-Prot Q5FHY3 (GUAC_LACAC)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LBA1893

Organism

Lactobacillus acidophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	GMP reductase HAMAP MF_01511		PRO_0000093758
Regions
	Nucleotide binding	209 – 232	24	NADP Potential
Sites
	Active site	180	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FHY3-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 3E8A176D3B43A018	FASTA	330	36,657
	10 20 30 40 50 60 MSNYFSMEAF DYDDIQLIPN KGIIKSRRDA DTSVKFGNRT FKIPVVPANM ESVIDDKLAV 70 80 90 100 110 120 WLAENDYYYV MHRFEPEKRI PFIKMMHEKG LFASISVGIK DSEYDFIDEL VKQNLKPEYI 130 140 150 160 170 180 TIDVAHGHSV YVIKMIKYIK EKLPNSFLTA GNIATPEAVR ELENAGADAT KVGVGPGKAC 190 200 210 220 230 240 ITKLKTGFGT GGWQLAALRM CSKVASKPLI ADGGIRHNGD IAKSVRFGAS MVMIGSMLAG 250 260 270 280 290 300 HEESPGNVIK IDGKTFKQYW GSASEVQKGA YKNVEGKQML VPYRGSIKDT LREMQEDLQS 310 320 330 AISYAGGREL NSIKLVDYVI VKDNIFDGDK

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCFM/NCK56/N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV43691.1.
RefSeq	YP_194722.1.
3D structure databases
SMR	Q5FHY3. Positions 10-325.
ModBase	Search...
Genome annotation databases
GeneID	3251822.
GenomeReviews	Gene locus LBA1893 in contig CP000033_GR.
KEGG	lac:LBA1893.
NMPDR	fig|272621.3.peg.1815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BioCyc	LACI272621:LBA1893-MONOMER.
BRENDA	1.7.1.7. 3045.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACAC

Accession

Primary (citable) accession number: Q5FHY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)