Reviewed, UniProtKB/Swiss-Prot Q5FHY0 (ASNA_LACAC)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	LBA1896

Organism

Lactobacillus acidophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1579 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017950

Sequences

	Sequence		Length	Mass (Da)
	Q5FHY0-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 7C8E15221AFD80B0	FASTA	336	38,744
	10 20 30 40 50 60 MKLILPKDYH PTLTVRDTEA AIVYIRETFQ DKLASILGVQ RMSAPMFVEK ASGLNDNLNG 70 80 90 100 110 120 VERPVGFDMK AIPDSEIEVV HSLAKWKRLA LKRYGFGMHE GLYTNMNAIR RDEDLDNFHS 130 140 150 160 170 180 IYVDQWDWEK IIAKEERNTD TLEVTVMKIF KAIKATEKLV TARYPGSTYR LGDHVSFITT 190 200 210 220 230 240 QELEDRWPEL SPEERENKIA KEEKAVFIMK IGDKLKRSGK PHDGRAPDYD DWQLNGDLIF 250 260 270 280 290 300 WYEPLQSKIE VSSMGIRVSE ESLREQLKKA HCEDREKLPF HKMLLEGKLP YTIGGGIGQS 310 320 330 RLCMLLLGKA HIGEVQASIW PEEMIEKCES DHIHLL

References

[1]

"Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM." Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S., Hamrick A., Cano R., Klaenhammer T.R. Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005) [PubMed: 15671160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCFM/NCK56/N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000033 Genomic DNA. Translation: AAV43694.1.
RefSeq	YP_194725.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
SMR	Q5FHY0. Positions 16-330.
ModBase	Search...
Genome annotation databases
GeneID	3251832.
GenomeReviews	Gene locus LBA1896 in contig CP000033_GR.
KEGG	lac:LBA1896.
NMPDR	fig|272621.3.peg.1818.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	LACI272621:LBA1896-MONOMER.
BRENDA	6.3.1.1. 3045.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACAC

Accession

Primary (citable) accession number: Q5FHY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)