Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Ehrlichia ruminantium (strain Gardel)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei595 – 5951ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciERUM302409:GHVW-318-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:ERGA_CDS_03010
OrganismiEhrlichia ruminantium (strain Gardel)
Taxonomic identifieri302409 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000000533 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Leucine--tRNA ligasePRO_0000334754Add
BLAST

Proteomic databases

PRIDEiQ5FHG5.

Interactioni

Protein-protein interaction databases

STRINGi302409.ERGA_CDS_03010.

Structurei

3D structure databases

ProteinModelPortaliQ5FHG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 4411"HIGH" regionAdd
BLAST
Motifi592 – 5965"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FHG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHYDFKEVER DVQEKWDFTV NVKDVQCYVL EMFPYPSGNI HMGHLRNYTI
60 70 80 90 100
GDVIARYKRA CGLHVFHPIG WDAFGLPAEN AALSYNISPQ VWTRKNIDNM
110 120 130 140 150
RSQLKSIGLS YNWDKEFATC DAEYYKYEQE FFLDFLKYGL AYRKESLVNW
160 170 180 190 200
DPVDQTVLAN EQVIDGRGWR SGAIIEKRKL SQWFLKITDF AAELLDDLKS
210 220 230 240 250
LNQWPEKVKL MQERWIGKSE GVIIDFQIVG INEVLQVFTT SPHTLFGASF
260 270 280 290 300
IAVSFDHPIL KYVNDVQLIQ LIDSFDRKDL IDDSSINTVE KFGINSGLVA
310 320 330 340 350
KHPLLDMDLP IYVANFVLMN YGTGAVFCCP AHDQRDFDFA KKYSLPIRQV
360 370 380 390 400
IFPEQNVDLE REAYVGSGIM GCSGFLDGMT VDDAKKSMIE KLISLGICKK
410 420 430 440 450
QVYYRLHDWG ISRQRYWGCP IPIIYCKKCG IVPVDKKDLP ITLPEDIDFT
460 470 480 490 500
KSGNPLDNHP TWKYTKCPSC GADAKRETDT FDTFFESSWY FAAFCGIGKG
510 520 530 540 550
IDKDVCNKLL PVDYYIGGIE HAVLHLLYSR FFCRALTRCG YFDVKEPFSS
560 570 580 590 600
LITQGMVCHS TYLDKYGNYL FPEDGKKMIQ EGKYVTVGRA EKMSKSKKNV
610 620 630 640 650
VHLDDIIDKY GADSARLFIL SDTPPERDIE WLDENIEGVS RYLNKLWKMI
660 670 680 690 700
VDYDQIEQNF VFNDISNDAV EYRMNVHKIL NDITNDLEFY RFNCAVAKFR
710 720 730 740 750
ELSNVISEMI RLSINHHVVS EAIYILIRVV EPFIPHIAEK LWQIVGGQGM
760 770 780 790 800
LCNQLWPKVD SQLLIKKNVN IVVQVNGKFI KAVSVPNDID DDTLKSIALE
810 820 830
VAQRRIGNSS VKNIYIIPAR VINIVITKSS
Length:830
Mass (Da):95,394
Last modified:March 1, 2005 - v1
Checksum:i39F0E588B3039040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI27753.1.
RefSeqiWP_011255458.1. NC_006831.1.
YP_196227.1. NC_006831.1.

Genome annotation databases

EnsemblBacteriaiCAI27753; CAI27753; ERGA_CDS_03010.
KEGGierg:ERGA_CDS_03010.
PATRICi20578312. VBIEhrRum72196_0320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI27753.1.
RefSeqiWP_011255458.1. NC_006831.1.
YP_196227.1. NC_006831.1.

3D structure databases

ProteinModelPortaliQ5FHG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi302409.ERGA_CDS_03010.

Proteomic databases

PRIDEiQ5FHG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI27753; CAI27753; ERGA_CDS_03010.
KEGGierg:ERGA_CDS_03010.
PATRICi20578312. VBIEhrRum72196_0320.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciERUM302409:GHVW-318-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Gardel.

Entry informationi

Entry nameiSYL_EHRRG
AccessioniPrimary (citable) accession number: Q5FHG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 1, 2005
Last modified: April 29, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.