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Q5FH91

- SYI_EHRRG

UniProt

Q5FH91 - SYI_EHRRG

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ehrlichia ruminantium (strain Gardel)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei650 – 6501ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciERUM302409:GHVW-527-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ERGA_CDS_05000
OrganismiEhrlichia ruminantium (strain Gardel)
Taxonomic identifieri302409 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000000533: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11181118Isoleucine--tRNA ligasePRO_0000098544Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi302409.ERGA_CDS_05000.

Structurei

3D structure databases

ProteinModelPortaliQ5FH91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi647 – 6515"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FH91-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCIVKTLHLI THVMKEYNSQ FTEGSDFSLI EEQILEFWKE NNIFKKSIEN
60 70 80 90 100
RDEKRRFVFY DGPPFANGLP HYGHLLTGFI KDTVARYKTM AGFRVDRRFG
110 120 130 140 150
WDCHGLPAEM LAEKELGVSG KLAIEEFGIE KFNNYCRNSV MKFSREWKQY
160 170 180 190 200
IDRQSRWVDF ENDYKTMNLS FMESIMWSFY QLWQKGLIYE SIKIVPYSWA
210 220 230 240 250
CQTPLSNFET RIDNAYRQKT SKTVTLAFEL LDAPKSLIVD NITAYKILVW
260 270 280 290 300
TTTPWTLPCN LALAISPNIK YCGAIINKEM YIFSRAYLKN FEDHCKKNNI
310 320 330 340 350
EYLIHSDDIC YLSLEYLSYK PVFNYFIDIK NAFKVLVADF VVEDEGTGIV
360 370 380 390 400
HMAPGFGEDD FILCKKQGIP DIDDKDTSKL LATICPIDDG GKFTERISDF
410 420 430 440 450
VNMHVFDTND QIISILKAKN LCFKTDQYLH NYPHCWRTDT PLIYRAMSSW
460 470 480 490 500
YVEVTKIKNR MIDLNKDVNW IPSHIRSGQF GKWLENAKDW AISRNRFWGT
510 520 530 540 550
PLPVWKSDNP NYPRIDVYGS IKKVFDDIKA LEEDFDIPID DLHRPYIDNL
560 570 580 590 600
VRPNPDDPTG KSMMRRVTDV FDCWFESGSM PYAQLHYPFE NKELFENYFP
610 620 630 640 650
ADFITEYVAQ TRGWFYTLFV LSTALFDKPP FKNCICHGVV LDTQGQKLSK
660 670 680 690 700
RLNNYADPME IFKQYGSDAM RFLMLSHTVS YGGDLLLDKD GVMVRDVIRN
710 720 730 740 750
VIKPMWNSYN FFTIYADIDK VSARVISDLD EVDNIMDKYI MCECISTIQS
760 770 780 790 800
IFNAMEEFDQ SVGNYGYNIK AACNSIVQFF EVLNNWYIRR CRSRFWSSEI
810 820 830 840 850
TKDKVNAYNT LYTVMYYMVK VSAPFLPAIT EAIWQKLNFQ EEESVHLSLL
860 870 880 890 900
PNIEHITLKD EDQKNIQYMK LIINICGCVL SIRNVRNIRV RQPLNKITIY
910 920 930 940 950
SYNNNNDLFN LPVKYQNILL DEINVKSIVF KSNIEDIASF QLKLNFPELG
960 970 980 990 1000
KRIPEKMKNL ISLLKSNQWK VLENGQLMLG IREGEYYILE DNEYTLNLKV
1010 1020 1030 1040 1050
HSEFASTITL GPNLLGVLVL DNTLTDELIM EGIARDIVRI IQQSRKDNKF
1060 1070 1080 1090 1100
NVSDKIDVVI CTQDKMVKNS VQAWYEYIVQ QTLSLSLVIH ENLDANNVAE
1110
YCKTTMKDRN LTLFIKKL
Length:1,118
Mass (Da):130,722
Last modified:December 20, 2005 - v2
Checksum:i1DB03A3249BFB06C
GO

Sequence cautioni

The sequence CAI27952.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR925677 Genomic DNA. Translation: CAI27952.1. Different initiation.
RefSeqiYP_196426.2. NC_006831.1.

Genome annotation databases

EnsemblBacteriaiCAI27952; CAI27952; ERGA_CDS_05000.
GeneIDi3268633.
KEGGierg:ERGA_CDS_05000.
PATRICi20578760. VBIEhrRum72196_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR925677 Genomic DNA. Translation: CAI27952.1 . Different initiation.
RefSeqi YP_196426.2. NC_006831.1.

3D structure databases

ProteinModelPortali Q5FH91.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 302409.ERGA_CDS_05000.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI27952 ; CAI27952 ; ERGA_CDS_05000 .
GeneIDi 3268633.
KEGGi erg:ERGA_CDS_05000.
PATRICi 20578760. VBIEhrRum72196_0535.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ERUM302409:GHVW-527-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Gardel.

Entry informationi

Entry nameiSYI_EHRRG
AccessioniPrimary (citable) accession number: Q5FH91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3