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Protein

Argininosuccinate synthase

Gene

argG

Organism
Ehrlichia ruminantium (strain Gardel)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei85CitrullineUniRule annotation1
Binding sitei90CitrullineUniRule annotation1
Binding sitei115ATP; via amide nitrogenUniRule annotation1
Binding sitei117AspartateUniRule annotation1
Binding sitei121AspartateUniRule annotation1
Binding sitei121CitrullineUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei125CitrullineUniRule annotation1
Binding sitei176CitrullineUniRule annotation1
Binding sitei185CitrullineUniRule annotation1
Binding sitei261CitrullineUniRule annotation1
Binding sitei273CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 15ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:ERGA_CDS_03850
OrganismiEhrlichia ruminantium (strain Gardel)
Taxonomic identifieri302409 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
Proteomesi
  • UP000000533 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002639251 – 394Argininosuccinate synthaseAdd BLAST394

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5FH27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FH27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIVLAYSG GLDTSVILKW LQENYNCEVV VFTADIGQED DMSVIQKKAA
60 70 80 90 100
ALNVKEIFIE DLKEEFVRDF VFPMFRANTI YEGYYLLGTS IARPLIAKRQ
110 120 130 140 150
IEIAHLTGAD AVAHGATGKG NDQVRFEFGY YCCDPNIKVI APWRQWELTS
160 170 180 190 200
RHSLIEYARK NNINVPLDKV NEPPYSIDAN LLHISYEGKS LEDPYVEPDY
210 220 230 240 250
TMLSRSLTPE LASSIPEYIE ITFEQGDPIA INDIPLSPAN LLHQLNKIGG
260 270 280 290 300
KHGIGIVDIV ENRYIGIKSR GIYETPGGTI LLHAHRAIES ITLDRESAHL
310 320 330 340 350
KDEIMPKYAK LIYNGYWWTT ERKMLQSLID NHKKKFNGTV RIKLYKGSVV
360 370 380 390
VVGRKSNNSL YSYNLASFDS ESQGYDHKDA EGFIKVNSLR LKKS
Length:394
Mass (Da):44,673
Last modified:March 1, 2005 - v1
Checksum:i6778BFEC73A33D1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI27837.1.
RefSeqiWP_011255525.1. NC_006831.1.

Genome annotation databases

EnsemblBacteriaiCAI27837; CAI27837; ERGA_CDS_03850.
KEGGierg:ERGA_CDS_03850.
PATRICi20578504. VBIEhrRum72196_0412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI27837.1.
RefSeqiWP_011255525.1. NC_006831.1.

3D structure databases

ProteinModelPortaliQ5FH27.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI27837; CAI27837; ERGA_CDS_03850.
KEGGierg:ERGA_CDS_03850.
PATRICi20578504. VBIEhrRum72196_0412.

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_EHRRG
AccessioniPrimary (citable) accession number: Q5FH27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.