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Q5FGW4 (SYE2_EHRRG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 2

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2
Gene names
Name:gltX2
Ordered Locus Names:ERGA_CDS_04420
OrganismEhrlichia ruminantium (strain Gardel) [Complete proteome] [HAMAP]
Taxonomic identifier302409 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022
PRO_0000119559

Regions

Motif11 – 2111"HIGH" region HAMAP-Rule MF_00022
Motif238 – 2425"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FGW4 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 2EB340613995DC37

FASTA47054,027
        10         20         30         40         50         60 
MLNHVVTRFA PSPTGHLHLG GARTALFNWL YAKHNNGKFL LRIEDTDSKR SSKELIDSII 

        70         80         90        100        110        120 
NSMIWLNIQH DGEIILQSSR ISRHIEIANQ LILNDKAYYC YCSEEEINLE KEQYTKKGLH 

       130        140        150        160        170        180 
YKHNCIWKNR KPPTGNCSKV IRLHSDTEGI TEFKDKVYGT IAVNNVQLDD MVLLRSNNTP 

       190        200        210        220        230        240 
TYLLSVVVDD YDMGITHIIR GTDHLTNTAR QLLIYNALGW KPPEFAHIPL IHDENGNKLS 

       250        260        270        280        290        300 
KRHHALGIHE YKNAGILPEA LVNYLLRMGW SHGNDELITI DEAIRWFSID KVGQSPAGLD 

       310        320        330        340        350        360 
SKKLEFLNNH YINTTNNATI IEMIIPIIEK TIGYKVNTEK IGYLLNGINE LKKRTKNLVN 

       370        380        390        400        410        420 
LANESLFYVE DIPISFDQES LIIIKSNHDI LSILYDNLSK VLNDDWNNST LTSMIKNIVK 

       430        440        450        460        470 
DYNTKIHNIY HCLRASIIGR INAPSIIDIM VNFQREECLN RIKYARDMVK 

« Hide

References

[1]"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Gardel.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR925677 Genomic DNA. Translation: CAI27894.1.
RefSeqYP_196368.1. NC_006831.1.

3D structure databases

ProteinModelPortalQ5FGW4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING302409.ERGA_CDS_04420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI27894; CAI27894; ERGA_CDS_04420.
GeneID3268757.
KEGGerg:ERGA_CDS_04420.
PATRIC20578630. VBIEhrRum72196_0473.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMAHCLRASI.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycERUM302409:GHVW-466-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE2_EHRRG
AccessionPrimary (citable) accession number: Q5FGW4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries