Q5FGB6 (SYE1_EHRRG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	ERGA_CDS_07930

Organism

Ehrlichia ruminantium (strain Gardel) [Complete proteome] [HAMAP]

Taxonomic identifier

302409 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000119558
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FGB6 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: EA69015D12BC3557	FASTA	445	51,819
	10 20 30 40 50 60 MIMMTRFAPS PTGYLHVGNV RTALICWLYT RSKQGRFLLR FDDTDLQRSK DDYRNEIAND 70 80 90 100 110 120 LKWLQMDWDF DVRQSSRFDR YDEIFNYLLK EELIYPCYES KEELDFKRKM QLKLGLPPIY 130 140 150 160 170 180 DRSALKLTQD EKNKYSEQDV YFRFKIDQSQ LISWDDEIRG KVSFNAANIS DPIVKRADGT 190 200 210 220 230 240 YTYMLPSVID DIDFDITHIV RGEDHISNTA IQIQMFNALR ASVPTFSHLS LLYCDDNKIS 250 260 270 280 290 300 KRVGGFSIKD MQFYELEPMA INSYFAKIGT SDPIVVHTKI QDLIYNFDIT KFNQAPTQFN 310 320 330 340 350 360 IDDVIKLNPK VLHKMSFSDV KHRLSELNIT SPDLWDFVSG NVQKFSDIQE WIKICGQSTV 370 380 390 400 410 420 PVINESDQDF IKMALSVFPN GEINQDTWKT WVANIKEKTD RKSKDIFIPL RLALTGISTG 430 440 PELAKLLPIL GRAEIIRRLG YSSRC

References

[1]

"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity." Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D. J. Bacteriol. 188:2533-2542(2006) [PubMed: 16547041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Gardel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR925677 Genomic DNA. Translation: CAI28245.1.
RefSeq	YP_196719.1. NC_006831.1.
3D structure databases
ProteinModelPortal	Q5FGB6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FGB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3268758.
GenomeReviews	Gene locus ERGA_CDS_07930 in contig CR925677_GR.
KEGG	erg:ERGA_CDS_07930.
PATRIC	20579402. VBIEhrRum72196_0848.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	ERUM302409:ERGA_CDS_07930-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_EHRRG

Accession

Primary (citable) accession number: Q5FGB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program