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Protein

Primosomal protein N'

Gene

priA

Organism
Ehrlichia ruminantium (strain Gardel)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi161 – 1688ATPUniRule annotation
Zinc fingeri377 – 38913C4-typeUniRule annotationAdd
BLAST
Zinc fingeri404 – 42017C4-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

HelicaseUniRule annotation, Hydrolase

Keywords - Biological processi

DNA replicationUniRule annotation

Keywords - Ligandi

ATP-binding, DNA-bindingUniRule annotation, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciERUM302409:GHVW-580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Primosomal protein N'UniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
ATP-dependent helicase PriAUniRule annotation
Gene namesi
Name:priAUniRule annotationImported
Ordered Locus Names:ERGA_CDS_05510Imported
OrganismiEhrlichia ruminantium (strain Gardel)Imported
Taxonomic identifieri302409 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000000533 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

PrimosomeUniRule annotation

Interactioni

Subunit structurei

Component of the primosome.UniRule annotation

Protein-protein interaction databases

STRINGi302409.ERGA_CDS_05510.

Structurei

3D structure databases

ProteinModelPortaliQ5FFP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 317170Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini412 – 568157Helicase C-terminalUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi260 – 2634DEAH boxUniRule annotation

Sequence similaritiesi

Belongs to the helicase family. PriA subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri377 – 38913C4-typeUniRule annotationAdd
BLAST
Zinc fingeri404 – 42017C4-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-fingerUniRule annotation

Phylogenomic databases

eggNOGiCOG1198.
HOGENOMiHOG000037414.
KOiK04066.
OMAiCHQCGET.
OrthoDBiEOG6KT2K4.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00983. PriA.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR005259. PriA.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00595. priA. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FFP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVSGMIAEV LLPVPVNKTF FYNIPYNENF IVGDYVIVPF GSRLIVGIVL
60 70 80 90 100
AISDSIPSTC EVNISQLKMV NCKGPLPGIC SALIHFIKWV SDYNVVPAGL
110 120 130 140 150
ILKMVFSNVV NVKFFNKLQF SVDENKKYSS DIKINLSDAQ KQAYNNIIGK
160 170 180 190 200
ISSYSVILLD GETGSGKTEV YCEVIRELVT RDNTAQVLVL LPEIVLTLQL
210 220 230 240 250
IKRIKYYFAN YFPVEWHSNL TVKSRREYWL SIAYGQSSIV IGARSALFLP
260 270 280 290 300
FKNLKMIIVD EEHDASFKQD YGVIYNARDM SIVLAKQFNI PVILSSATPS
310 320 330 340 350
LETISNIQKS QYYHVRLTQR FGDARLPTVK IIDLCKSKMV NRWLSYDLYS
360 370 380 390 400
SMLEVLQRQD QVMLFLNRRG YATLRLCKTC GFKVQCKNCA TWLIEHKRRN
410 420 430 440 450
VLLCHYCGYS CTIVDECYNC LDKSSVISYG IGVEKVAEDI MTLIPDAKIA
460 470 480 490 500
IISSDISSKE IGVTIDLIMQ NKVNIIIGTQ VIAKGHNFPK LTLVGVIDAD
510 520 530 540 550
LGLNNSDLRA TERTYQLLHQ VAGRSGRFVD RSEVILQTYD ANNALIKSLS
560 570 580 590 600
SYNRELFYDL ELKSRLLTQM PPYTRLIGII VTGKNEAKVI NVSQQIVKSL
610 620 630 640 650
PTTLTVLGPA PAPISLLNRQ YRYRILIKVQ NNVVIQKLLT RYKEYYASTG
660
KVKIIIDVDP INFM
Length:664
Mass (Da):75,100
Last modified:March 1, 2005 - v1
Checksum:i63F1AA35CA1C3C25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI28003.1.
RefSeqiWP_011255664.1. NC_006831.1.
YP_196477.1. NC_006831.1.

Genome annotation databases

EnsemblBacteriaiCAI28003; CAI28003; ERGA_CDS_05510.
KEGGierg:ERGA_CDS_05510.
PATRICi20578864. VBIEhrRum72196_0585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925677 Genomic DNA. Translation: CAI28003.1.
RefSeqiWP_011255664.1. NC_006831.1.
YP_196477.1. NC_006831.1.

3D structure databases

ProteinModelPortaliQ5FFP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi302409.ERGA_CDS_05510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI28003; CAI28003; ERGA_CDS_05510.
KEGGierg:ERGA_CDS_05510.
PATRICi20578864. VBIEhrRum72196_0585.

Phylogenomic databases

eggNOGiCOG1198.
HOGENOMiHOG000037414.
KOiK04066.
OMAiCHQCGET.
OrthoDBiEOG6KT2K4.

Enzyme and pathway databases

BioCyciERUM302409:GHVW-580-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00983. PriA.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR005259. PriA.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00595. priA. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassami Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GardelImported.

Entry informationi

Entry nameiQ5FFP0_EHRRG
AccessioniPrimary (citable) accession number: Q5FFP0
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 29, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.