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Q5FFK4

- LIPA_EHRRG

UniProt

Q5FFK4 - LIPA_EHRRG

Protein

Lipoyl synthase

Gene

lipA

Organism
Ehrlichia ruminantium (strain Gardel)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciERUM302409:GHVW-573-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:ERGA_CDS_05440
    OrganismiEhrlichia ruminantium (strain Gardel)
    Taxonomic identifieri302409 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
    ProteomesiUP000000533: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Lipoyl synthasePRO_1000012218Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi302409.ERGA_CDS_05440.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FFK4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5FFK4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSKPDWLKV KMPTGSAFYE MRNLMKLHKL NTVCEEAACP NIGECWNKKH    50
    ATVMILGSTC TRACAFCNVA SGIPDKLDPH EPQSLAKAVS SLKLQHVVIT 100
    SVDRDDLEDG GAGHFVECIE EIRKRDSNVT IEILTPDFLN KHDAIDKIAK 150
    AFPDVYNHNV ETVPRLYAKI RPKARYFHSL YLLKTIKQKN PRIFTKSGIM 200
    VGLGELKEEI YQVMDDLRSA DVDFIVIGQY LQPTSKHAVV DRYVTPEEFD 250
    HYKYVAYSKG FLMVASGPLV RSSYHAEEDF QRLKKNRAAM YT 292
    Length:292
    Mass (Da):33,219
    Last modified:March 1, 2005 - v1
    Checksum:i0255F7BAF478899C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR925677 Genomic DNA. Translation: CAI27996.1.
    RefSeqiWP_011155209.1. NC_006831.1.
    YP_196470.1. NC_006831.1.

    Genome annotation databases

    EnsemblBacteriaiCAI27996; CAI27996; ERGA_CDS_05440.
    GeneIDi3268613.
    KEGGierg:ERGA_CDS_05440.
    PATRICi20578850. VBIEhrRum72196_0578.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR925677 Genomic DNA. Translation: CAI27996.1 .
    RefSeqi WP_011155209.1. NC_006831.1.
    YP_196470.1. NC_006831.1.

    3D structure databases

    ProteinModelPortali Q5FFK4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 302409.ERGA_CDS_05440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI27996 ; CAI27996 ; ERGA_CDS_05440 .
    GeneIDi 3268613.
    KEGGi erg:ERGA_CDS_05440.
    PATRICi 20578850. VBIEhrRum72196_0578.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci ERUM302409:GHVW-573-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
      Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
      J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Gardel.

    Entry informationi

    Entry nameiLIPA_EHRRG
    AccessioniPrimary (citable) accession number: Q5FFK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3