ID   SYL_NEIG1               Reviewed;         876 AA.
AC   Q5FAJ3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   27-MAR-2024, entry version 130.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   OrderedLocusNames=NGO0006;
OS   Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=242231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700825 / FA 1090;
RA   Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F.,
RA   Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C.,
RA   Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H., Qi S.,
RA   Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W.;
RT   "The complete genome sequence of Neisseria gonorrhoeae.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AE004969; AAW88778.2; -; Genomic_DNA.
DR   RefSeq; WP_025456399.1; NC_002946.2.
DR   RefSeq; YP_207190.2; NC_002946.2.
DR   PDB; 6YKK; X-ray; 2.24 A; A=1-876.
DR   PDB; 6YKL; X-ray; 2.27 A; A=1-876.
DR   PDB; 6YKN; X-ray; 2.63 A; A=1-876.
DR   PDB; 6YKO; X-ray; 2.21 A; A=1-876.
DR   PDB; 6YKQ; X-ray; 1.94 A; A=1-876.
DR   PDB; 6YKS; X-ray; 1.97 A; A=1-876.
DR   PDB; 6YKT; X-ray; 2.32 A; A=1-876.
DR   PDB; 6YKU; X-ray; 2.14 A; A=1-876.
DR   PDB; 6YKV; X-ray; 2.43 A; A=1-876.
DR   PDB; 6YKW; X-ray; 2.46 A; A=1-876.
DR   PDB; 6YKX; X-ray; 2.41 A; A=1-876.
DR   PDB; 7A0P; X-ray; 2.18 A; A=1-876.
DR   PDB; 7AP2; X-ray; 2.25 A; A=1-876.
DR   PDBsum; 6YKK; -.
DR   PDBsum; 6YKL; -.
DR   PDBsum; 6YKN; -.
DR   PDBsum; 6YKO; -.
DR   PDBsum; 6YKQ; -.
DR   PDBsum; 6YKS; -.
DR   PDBsum; 6YKT; -.
DR   PDBsum; 6YKU; -.
DR   PDBsum; 6YKV; -.
DR   PDBsum; 6YKW; -.
DR   PDBsum; 6YKX; -.
DR   PDBsum; 7A0P; -.
DR   PDBsum; 7AP2; -.
DR   AlphaFoldDB; Q5FAJ3; -.
DR   SMR; Q5FAJ3; -.
DR   STRING; 242231.NGO_0006; -.
DR   KEGG; ngo:NGO_0006; -.
DR   PATRIC; fig|242231.10.peg.7; -.
DR   HOGENOM; CLU_004427_0_0_4; -.
DR   Proteomes; UP000000535; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd07958; Anticodon_Ia_Leu_BEm; 1.
DR   CDD; cd00812; LeuRS_core; 1.
DR   Gene3D; 2.20.28.290; -; 1.
DR   Gene3D; 3.10.20.590; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00396; leuS_bact; 1.
DR   PANTHER; PTHR43740:SF2; LEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43740; LEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 2.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..876
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334780"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           634..638
FT                   /note="'KMSKS' region"
FT   BINDING         637
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
FT   HELIX           7..21
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            22..24
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          30..32
FT                   /evidence="ECO:0007829|PDB:6YKV"
FT   STRAND          34..39
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          45..47
FT                   /evidence="ECO:0007829|PDB:6YKV"
FT   HELIX           50..68
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          72..74
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           84..93
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           97..114
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           121..123
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           130..145
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          148..159
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            160..163
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          164..166
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           168..170
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            177..179
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          184..193
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           195..198
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           199..204
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           205..208
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           214..224
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          226..236
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           238..240
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           246..249
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          251..257
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           259..264
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          267..270
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           275..281
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           285..296
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           301..304
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          311..319
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            321..323
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          326..332
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          342..346
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            348..350
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           352..361
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          378..380
FT                   /evidence="ECO:0007829|PDB:6YKL"
FT   HELIX           383..386
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          388..390
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           397..399
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           404..417
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          420..427
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          435..437
FT                   /evidence="ECO:0007829|PDB:6YKL"
FT   STRAND          439..441
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          446..449
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            450..452
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          453..456
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           459..461
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          474..476
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           478..481
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           483..486
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          487..489
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            491..493
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          496..499
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           507..510
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           513..516
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          523..527
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           529..535
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          537..544
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           545..547
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           550..564
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          573..580
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          583..591
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          593..595
FT                   /evidence="ECO:0007829|PDB:7A0P"
FT   STRAND          597..600
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           602..604
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          605..607
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          618..620
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            621..623
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          626..634
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            637..640
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           645..652
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           654..664
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          667..669
FT                   /evidence="ECO:0007829|PDB:6YKV"
FT   HELIX           675..697
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           714..735
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           740..755
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           762..779
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            780..782
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           784..794
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           799..802
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           809..812
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          820..823
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   HELIX           841..847
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          865..867
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   TURN            868..870
FT                   /evidence="ECO:0007829|PDB:6YKQ"
FT   STRAND          871..874
FT                   /evidence="ECO:0007829|PDB:6YKQ"
SQ   SEQUENCE   876 AA;  98049 MW;  E2B73DC55263D03E CRC64;
     MQEHYQPAAI EPAAQKKWDD ARISNVSEDA SKPKYYCLSM FPYPSGKLHM GHVRNYTIGD
     VLSRFKLLNG FNVMQPMGWD AFGMPAENAA MKNNVAPAAW TYDNIEYMKT QLKSLGFAID
     WEREVATCKP EYYRWEQWLF TKLFEKGIVY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG
     ALIEKREIPM YYFKITDYAE ELLNDLDKLE HWPEQVKTMQ RNWIGKSRGM TVRFAVSDDS
     KQGLEGDYAK FLQVYTTRPD TLMGATYVAV AAEHPLATAA AADKPELQAF IAECKAGSVA
     EADMATMEKK GVPTGRYVVN PLNGDKLEVW IANYVLWGYG DGAVMAVPAH DERDFEFAAK
     YNLPKKQVIA VGDNAFDANR WQEWYGDKEN GVLVNSGDLD GLDFQTAFDA VAAKLQSQGA
     GEPKTQYRLR DWGISRQRYW GCPIPIVHCE KCGDVPVPAD QLPVVLPENV VPDGMGSPLA
     KMPEFYETSC PCCGGAAKRE TDTMDTFMES SWYFFRYMSP KFSDGMVSAE SAKYWGAVDQ
     YIGGIEHAIL HLLYARFFTK LMRDEGLVNV DEPFERLLTQ GMVVCETYYR ENDKGGKDWI
     NPADVELTFD DKGRPVSAVL KADGLPVVIS GTEKMSKSKN NGVDPQELIN AYGADTARLF
     MMFAAPPEQS LEWSDSGVEG AHRFLRRLWR TVYEYLKQGG AVKAFAGNQD GLSKELKDLR
     HKLHSTTAKV SDDYGRRQQF NTAIAAVMEL LNQYDKTDTG SEQGRAVAQE VLEAAVRLLW
     PIVPHICETL WSELNGAKLW EAGWPTVDEA ALVKSEIEVM VQVNGKLRGK ITVAADASKA
     DLEAAALANE GAVKFMEGKP AKKIIVVPGR LVNIVV
//