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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[3Fe-4S] clusterUniRule annotationNote: Binds 1 [3Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei44 – 441SubstrateUniRule annotation
Binding sitei77 – 771SubstrateUniRule annotation
Metal bindingi99 – 991Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei127 – 1271SubstrateUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Metal bindingi198 – 1981Iron-sulfur (3Fe-4S)UniRule annotation
Binding sitei270 – 2701SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciNGON242231:GI2G-64-MONOMER.
UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Gene namesi
Name:ispHUniRule annotation
Ordered Locus Names:NGO0072
OrganismiNeisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Taxonomic identifieri242231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000000535 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3223224-hydroxy-3-methylbut-2-enyl diphosphate reductasePRO_0000128843Add
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni226 – 2283Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220192.
KOiK03527.
OMAiDDLTFMT.
OrthoDBiEOG6HF624.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5FAF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKTIILAN PRGFCAGVDR AISIVERALE EFGAPVYVRH EVVHNKFVVD
60 70 80 90 100
NLREKGAVFI EDLAEVPPGA TLVYSAHGVS KAVQQEAAER GFRVFDATCP
110 120 130 140 150
LVTKVHKEVA RLDAQDCEII MIGHKGHAEV EGTMGQLAPG KMLLVETVGD
160 170 180 190 200
VAKLEVRNPD KLAYVSQTTL SVDETKDIIA ALNARFPNIR NPHKEDICYA
210 220 230 240 250
TTNRQTAVKE LAEQCDIVIV VGSPNSSNSN RLREVAASRG IDAYMVDNAS
260 270 280 290 300
YLQRTWFEGK SKVGVTAGAS APEVLVREVL ATIRGWGHET VREGGGAEES
310 320
IVFVLPKELR REGETKPDLC KR
Length:322
Mass (Da):35,141
Last modified:March 15, 2005 - v1
Checksum:i8C41368DF0033B13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004969 Genomic DNA. Translation: AAW88835.1.
RefSeqiWP_010356621.1. NC_002946.2.
YP_207247.1. NC_002946.2.

Genome annotation databases

EnsemblBacteriaiAAW88835; AAW88835; NGO_0072.
GeneIDi3282290.
KEGGingo:NGO0072.
PATRICi20333018. VBINeiGon24812_0093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004969 Genomic DNA. Translation: AAW88835.1.
RefSeqiWP_010356621.1. NC_002946.2.
YP_207247.1. NC_002946.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

DNASUi3282290.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW88835; AAW88835; NGO_0072.
GeneIDi3282290.
KEGGingo:NGO0072.
PATRICi20333018. VBINeiGon24812_0093.

Phylogenomic databases

HOGENOMiHOG000220192.
KOiK03527.
OMAiDDLTFMT.
OrthoDBiEOG6HF624.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.
BioCyciNGON242231:GI2G-64-MONOMER.

Family and domain databases

HAMAPiMF_00191. IspH.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Neisseria gonorrhoeae."
    Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H.
    , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700825 / FA 1090.

Entry informationi

Entry nameiISPH_NEIG1
AccessioniPrimary (citable) accession number: Q5FAF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 15, 2005
Last modified: May 11, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.