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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei44SubstrateUniRule annotation1
Binding sitei77SubstrateUniRule annotation1
Metal bindingi99Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei127SubstrateUniRule annotation1
Active sitei129Proton donorUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Metal bindingi198Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei270SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNGON242231:G1FZ9-66-MONOMER
UniPathwayiUPA00056; UER00097
UPA00059; UER00105

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Ordered Locus Names:NGO0072
OrganismiNeisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Taxonomic identifieri242231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000000535 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001288431 – 3224-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST322

Proteomic databases

PRIDEiQ5FAF2

Structurei

3D structure databases

SMRiQ5FAF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni226 – 228Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220192
KOiK03527
OMAiHNKYVVD

Family and domain databases

CDDicd13944 lytB_ispH, 1 hit
HAMAPiMF_00191 IspH, 1 hit
InterProiView protein in InterPro
IPR003451 LytB/IspH
PANTHERiPTHR30426 PTHR30426, 1 hit
PfamiView protein in Pfam
PF02401 LYTB, 1 hit
TIGRFAMsiTIGR00216 ispH_lytB, 1 hit

Sequencei

Sequence statusi: Complete.

Q5FAF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKTIILAN PRGFCAGVDR AISIVERALE EFGAPVYVRH EVVHNKFVVD
60 70 80 90 100
NLREKGAVFI EDLAEVPPGA TLVYSAHGVS KAVQQEAAER GFRVFDATCP
110 120 130 140 150
LVTKVHKEVA RLDAQDCEII MIGHKGHAEV EGTMGQLAPG KMLLVETVGD
160 170 180 190 200
VAKLEVRNPD KLAYVSQTTL SVDETKDIIA ALNARFPNIR NPHKEDICYA
210 220 230 240 250
TTNRQTAVKE LAEQCDIVIV VGSPNSSNSN RLREVAASRG IDAYMVDNAS
260 270 280 290 300
YLQRTWFEGK SKVGVTAGAS APEVLVREVL ATIRGWGHET VREGGGAEES
310 320
IVFVLPKELR REGETKPDLC KR
Length:322
Mass (Da):35,141
Last modified:March 15, 2005 - v1
Checksum:i8C41368DF0033B13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004969 Genomic DNA Translation: AAW88835.1
RefSeqiWP_010356621.1, NC_002946.2
YP_207247.1, NC_002946.2

Genome annotation databases

EnsemblBacteriaiAAW88835; AAW88835; NGO_0072
GeneIDi3282290
KEGGingo:NGO0072
PATRICifig|242231.10.peg.93

Similar proteinsi

Entry informationi

Entry nameiISPH_NEIG1
AccessioniPrimary (citable) accession number: Q5FAF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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