Q5F9X9 (RISB_NEIG1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	NGO0257

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040461

Sequences

	Sequence		Length	Mass (Da)
	Q5F9X9 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 0CA5502CB5690093	FASTA	158	16,968
	10 20 30 40 50 60 MNTIAPNLDG KHLRIGIVQA RFTNEIGSQM LKVCCRTLQE LGVADENITV ATVPGALEIP 70 80 90 100 110 120 IALMNFASSE KFDALIAIGV VIRGETYHFE LVANESGAGI GRVALDYNIP IANAVLTTEN 130 140 150 DAQAIERIGE KASDAAKVAV ECANLVNLLL EEQFEDEE

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700825 / FA 1090.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004969 Genomic DNA. Translation: AAW89008.1.
RefSeq	YP_207420.1. NC_002946.2.
3D structure databases
HSSP	HSSP built from PDB template 1C41 based on UniProtKB Q9UVT8.
ProteinModelPortal	Q5F9X9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F9X9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000003891; EBNEIP00000003732; EBNEIG00000003891.
GeneID	3281556.
GenomeReviews	Gene locus NGO0257 in contig AE004969_GR.
KEGG	ngo:NGO0257.
NMPDR	fig|242231.4.peg.375.
PATRIC	20333471. VBINeiGon24812_0316.
Phylogenomic databases
eggNOG	COG0054.
GeneTree	EBGT00050000020812.
HOGENOM	HBG311126.
OMA	IQGATKH.
PhylomeDB	Q5F9X9.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	NGON242231:NGO0257-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_NEIG1

Accession

Primary (citable) accession number: Q5F9X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program