Q5F8T3 (LEUD_NEIG1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase

Gene names

Name:	leuD
Ordered Locus Names:	NGO0677

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) [Reference proteome] [HAMAP]

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria gonorrhoeae

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01031
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01031
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01031
Subunit structure	Heterodimer of LeuC and LeuD By similarity.
Sequence similarities	Belongs to the LeuD family. LeuD type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	3-isopropylmalate dehydratase small subunit HAMAP-Rule MF_01031		PRO_0000141842

Sequences

	Sequence		Length	Mass (Da)
	Q5F8T3 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 0835E832C3FE3C82	FASTA	213	24,208
	10 20 30 40 50 60 MKAFTKITAI VAPLDRSNVD TDAIIPKQFL KSIKRSGFGP NAFDEWRYLD HGEPGMDNGK 70 80 90 100 110 120 RPLNPDFSLN QPRYQGAQIL LTRKNFGCGS SREHAPWALD DYGFRAIIAP SFADIFFNNC 130 140 150 160 170 180 YKNGLLPIVL TEEQVDRLFK EVEANEGYRL SIDLAEQTLT TPGGETFTFD ITEHRKHCLL 190 200 210 NGLDEIGLTL QHADKIKAFE EKRRQSQPWL FNG

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700825 / FA 1090.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004969 Genomic DNA. Translation: AAW89404.1.
RefSeq	YP_207816.1. NC_002946.2.
3D structure databases
ProteinModelPortal	Q5F8T3.
ModBase	Search...
Protein-protein interaction databases
STRING	242231.NGO0677.
Proteomic databases
PRIDE	Q5F8T3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW89404; AAW89404; NGO0677.
GeneID	3282001.
KEGG	ngo:NGO0677.
PATRIC	20334452. VBINeiGon24812_0798.
Phylogenomic databases
eggNOG	COG0066.
HOGENOM	HOG000222939.
KO	K01704.
OMA	YQDNSKR.
ProtClustDB	PRK01641.
Enzyme and pathway databases
BioCyc	NGON242231:GI2G-632-MONOMER.
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.20.19.10. 1 hit.
HAMAP	MF_01031. LeuD_type1.
InterPro	IPR004431. 3-IsopropMal_deHydase_ssu. IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF2. PTHR11670:SF2. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00171. leuD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD_NEIG1

Accession

Primary (citable) accession number: Q5F8T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program