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Protein

Antitoxin FitA

Gene

fitA

Organism
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Plays a role in the speed with which bacteria traverse human epithelial cells; disruption of the locus increases the speed of trafficking about 2-4-fold. Binds to its own promoter, binding affinity of the FitAB complex is 20-30-fold higher than FitA alone. No nuclease activity was observed for the FitAB complex, perhaps because FitA (the antitoxin) prevents metal binding and thus catalysis by FitB.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • migration in host Source: UniProtKB
  • regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin FitA
Alternative name(s):
Trafficking protein A
Gene namesi
Name:fitA
Synonyms:vapB
Ordered Locus Names:NGO0908
OrganismiNeisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Taxonomic identifieri242231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000000535 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Disruption of the fitAB operon leads to faster transepithelial cell trafficking of the bacterium; mutants adhere to and invade cells normally. Mutants grow normally in liquid culture but much faster within human cell lines A431 and T84; these latter 2 phenotypes were observed using MS11A bacteria with a disrupted fitAB locus.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7R → A: Loss of DNA-binding, still binds FitB. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004080861 – 78Antitoxin FitAAdd BLAST78

Interactioni

Subunit structurei

Homodimer in the absence of FitB; forms a heterodimer with FitB; 4 FitAB heterodimers form a complex that binds to fitAB promoter DNA. The complex is also seen in solution.2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Structurei

Secondary structure

178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi11 – 23Combined sources13
Helixi28 – 43Combined sources16
Helixi48 – 59Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BSQX-ray3.00E/F/G/H2-78[»]
2H1CX-ray1.80B46-64[»]
2H1OX-ray3.00E/F/G/H2-69[»]
ProteinModelPortaliQ5F881.
SMRiQ5F881.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5F881.

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000142203.
OMAiCIMPVIT.

Family and domain databases

Gene3Di1.10.1220.10. 1 hit.
InterProiIPR013321. Arc_rbn_hlx_hlx.
IPR010985. Ribbon_hlx_hlx.
[Graphical view]
SUPFAMiSSF47598. SSF47598. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5F881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVVIRNLS EATHNAIKFR ARAAGRSTEA EIRLILDNIA KAQQTVRLGS
60 70
MLASIGQEIG GVELEDVRGR NTDNEVSL
Length:78
Mass (Da):8,434
Last modified:March 15, 2005 - v1
Checksum:i29CC28FF3DCD9C73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200716 Genomic DNA. Translation: AAF19188.1.
AE004969 Genomic DNA. Translation: AAW89606.1.
RefSeqiWP_003688410.1. NC_002946.2.
YP_208018.1. NC_002946.2.

Genome annotation databases

EnsemblBacteriaiAAW89606; AAW89606; NGO_0908.
GeneIDi3281660.
KEGGingo:NGO_0908.
PATRICi20334997. VBINeiGon24812_1068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200716 Genomic DNA. Translation: AAF19188.1.
AE004969 Genomic DNA. Translation: AAW89606.1.
RefSeqiWP_003688410.1. NC_002946.2.
YP_208018.1. NC_002946.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BSQX-ray3.00E/F/G/H2-78[»]
2H1CX-ray1.80B46-64[»]
2H1OX-ray3.00E/F/G/H2-69[»]
ProteinModelPortaliQ5F881.
SMRiQ5F881.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW89606; AAW89606; NGO_0908.
GeneIDi3281660.
KEGGingo:NGO_0908.
PATRICi20334997. VBINeiGon24812_1068.

Phylogenomic databases

HOGENOMiHOG000142203.
OMAiCIMPVIT.

Miscellaneous databases

EvolutionaryTraceiQ5F881.

Family and domain databases

Gene3Di1.10.1220.10. 1 hit.
InterProiIPR013321. Arc_rbn_hlx_hlx.
IPR010985. Ribbon_hlx_hlx.
[Graphical view]
SUPFAMiSSF47598. SSF47598. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFITA_NEIG1
AccessioniPrimary (citable) accession number: Q5F881
Secondary accession number(s): Q9RF92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.