Q5F863 (METE_NEIG1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	NGO0928

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017257
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F863 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1EF1567F4AA09850	FASTA	758	85,013
	10 20 30 40 50 60 MTTLHFSGFP RVGAFRELKF AQEKYWRKEI SEQELLDVAK DLREKNWKHQ AAANADYVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQNL PLEQFFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFHADTE FKANAKHYVQ QLQEAQTLGL KAKPTVVGPL TFLWVGKEKG SVEFDRLSLL 190 200 210 220 230 240 PKLLPVYVEI LTALVEAGAE WIQIDEPALA VDLPKEWVEA YKDVYATLNK VSAKILLGTY 250 260 270 280 290 300 FGSVAEHAAL LKSLPVDGLH IDLVRAPEQL DAFAGYDKVL SAGVIDGRNI WRANLNKVLE 310 320 330 340 350 360 TVGPLQAKLG ERLWISSSCS LLHTPFDLSV EEKLKANKPD LYSWLAFTLQ KTQELRVLKA 370 380 390 400 410 420 ALNEGRDSVA EELAASQAAA DSRANSSEIH RADVAKRLAD LPVNAGQRKS PFADRIKAQQ 430 440 450 460 470 480 AWLNLPLLPT TNIGSFPQTT EIRQARAAFK KGELSASDYE AAMKKEIALV VEEQEKLDLD 490 500 510 520 530 540 VLVHGEAERN DMVEYFGELL SGFAFTQYGW VQSYGSRCVK PPIIFGDVSR PEAMTVAWST 550 560 570 580 590 600 YAQNLTKRPM KGMLTGPVTI LQWSFVRNDI PRATVCKQIA LALNDEVLDL EKAGIKVIQI 610 620 630 640 650 660 DEPAIREGLP LKRADWDAYL NWAGESFRLS SAGCEDSTQI HTHMCYSEFN DILPAIAAMD 670 680 690 700 710 720 ADVITIETSR SDMELLAAFG EFKYPNDIGP GVYDIHSPRV PTEAEVEHLL RKAIEVVPVE 730 740 750 RLWVNPDCGL KTRGWKETLE QLQVMMNVTR KLRAELAK

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700825 / FA 1090.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004969 Genomic DNA. Translation: AAW89624.1.
RefSeq	YP_208036.1. NC_002946.2.
3D structure databases
HSSP	HSSP built from PDB template 1U1J based on UniProtKB O50008.
ProteinModelPortal	Q5F863.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000003692; EBNEIP00000003533; EBNEIG00000003692.
GeneID	3282587.
GenomeReviews	Gene locus NGO0928 in contig AE004969_GR.
KEGG	ngo:NGO0928.
NMPDR	fig|242231.4.peg.407.
PATRIC	20335039. VBINeiGon24812_1089.
Phylogenomic databases
eggNOG	COG0620.
GeneTree	EBGT00050000021045.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q5F863.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	NGON242231:NGO0928-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_NEIG1

Accession

Primary (citable) accession number: Q5F863

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program