Reviewed, UniProtKB/Swiss-Prot Q5F7J4 (PUR4_NEIG1)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	NGO1183

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) [Complete proteome] [HAMAP]

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	1314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1314	1314	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264582
Regions
	Domain	1063 – 1314	252	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1156	1	Nucleophile By similarity
	Active site	1279	1	By similarity
	Active site	1281	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F7J4-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: B9DD36708873144D	FASTA	1,314	142,972
	10 20 30 40 50 60 MPLRGVTALS DFRVEKLLQK AAALGLPEVK LSSEFWYFAG SEKALDAATV EKLQALLAAQ 70 80 90 100 110 120 SVEQTPEARE GLHLFLVTPR LGTISPWASK ATNIAENCGL AGIERIERGM AVWLEGALTD 130 140 150 160 170 180 EQKQQWAALL HDRMTESVLP DFQTASKLFH HLKSETFSTV DVLGGGKEAL VKANTETGLA 190 200 210 220 230 240 LSADEIDYLV ENYQALQRNP SDVELMMFAQ ANSEHCRHKI FNADFILNGE KQPKSLFGMI 250 260 270 280 290 300 RDTHNAHPEG TVVAYKDNSS VIKGAKIERF YPNAAENQGY RFHEEDTHII MKVETHNHPT 310 320 330 340 350 360 AIAPFAGAAT GAGGEIRDEG ATGKGSRPKA GLTGFTVSNL NIPDLKQPWE QDYGKPEHIS 370 380 390 400 410 420 SPLDIMIEGP IGGAAFNNEF GRPNLLGYFR TFEEKFDGQV RGYHKPIMIA GGLGSIQAQQ 430 440 450 460 470 480 THKDEIPEGA LLIQLGGPGM LIGLGGGAAS SMDTGTNDAS LDFNSVQRGN PEIERRAQEV 490 500 510 520 530 540 IDRCWQLGDQ NPIISIHDVG AGGLSNAFPE LVNDAGRGAV FELREVPLEE HGLTPLQIWC 550 560 570 580 590 600 NESQERYVLS ILEKDLDTFR AICERERCPF AVVGTATDDG HLKVRDDLFS NNPVDLPLNV 610 620 630 640 650 660 LLGKPPKTTR TDKTVTPSKK PFHAGDIDIT EAAYRVLRLP AVAAKNFLIT IGDRSVGGMT 670 680 690 700 710 720 HRDQMVGKYQ TPVADCAVTM MGFNTYRGEA MSMGEKPAVA LFDAPASGRM CVGEAITNIA 730 740 750 760 770 780 AVNIGDIGNI KLSANWMAAC GNEGEDEKLY RTVEAVSKAC QALDLSIPVG KDSLSMKTVW 790 800 810 820 830 840 QDGEEKKSVV SPLSLIISAF APVKDVRKTV TPELKNVEGS VLLFIDLGFG KARMGGSAFG 850 860 870 880 890 900 QVYNNMSGDA PDLDDAGRLK AFYSVIQQLV AEDKLLAYHD RSDGGLFATL AEMAFAARCG 910 920 930 940 950 960 ISADIDCLMD KFLPIHLPDF QGDPAEDLSD ELYNHAAIKI LFNEELGAVI QIRQKDRDYV 970 980 990 1000 1010 1020 DAAFETAGLT DAVSRIGSPD FDNEFISFFG YGYFLEQNRA DLQRAWQETS HAIQRLRDNP 1030 1040 1050 1060 1070 1080 ACADSEFALI GDNERSALFA DVKFDVNEDI AAPFINSGAK PKIAILREQG VNGQIEMAAA 1090 1100 1110 1120 1130 1140 FTRAGFDAYD VHMSDLMAGR FRLADFKMLA ACGGFSYGDV LGAGEGWAKS ILFHPALRDQ 1150 1160 1170 1180 1190 1200 FAAFFADPNT LTLGVCNGCQ MVSNLAEIIP GAETWPKFKR NLSEQFEARL NMVHVPKSAS 1210 1220 1230 1240 1250 1260 LILNEMQDSS LPVVVSHGEG RADFALHGGN ISADLGIALQ YVDGQNQVTQ TYPLNPNGSP 1270 1280 1290 1300 1310 QGIAGITNAD GRVTIMMPHP ERVYRAAQMS RKPEGWTELS GWYRLFAGAR KALG

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004969 Genomic DNA. Translation: AAW89843.1. Different initiation.
RefSeq	YP_208255.1.
3D structure databases
HSSP	HSSP built from PDB template 1T3T based on UniProtKB P74881.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F7J4.
Genome annotation databases
GeneID	3281977.
GenomeReviews	Gene locus NGO1183 in contig AE004969_GR.
KEGG	ngo:NGO1183.
NMPDR	fig|242231.4.peg.1042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG335309.
OMA	ERGIAYY.
PhylomeDB	Q5F7J4.
Enzyme and pathway databases
BioCyc	NGON242231:NGO1183-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_NEIG1

Accession

Primary (citable) accession number: Q5F7J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)