Q5F7G0 (SYQ_NEIG1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	NGO1218

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) [Reference proteome] [HAMAP]

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria gonorrhoeae

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00126
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000242871
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	271 – 275	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F7G0 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 0E28E106DAF63762	FASTA	562	64,572
	10 20 30 40 50 60 MLNKDQFADN HFIRTIIEDD LKSGKHEAVQ TRFPPEPNGY LHIGHAKSIC LNFGLAYIYD 70 80 90 100 110 120 GLCNLRFDDT NPEKENDEYV NAIKEDVEWL GFHWAGEPRF ASDYFDRLYD YAVGLIKDGK 130 140 150 160 170 180 AYVDDLTPEE MREYRGTLTE AGKNSPYRDR SIEENLDLFT RMKNGEFPDG SKTLRLKIDM 190 200 210 220 230 240 AAGNINMRDP VIYRIRRAHH HNTGDKWCIY PMYDYTHCIS DAIEGITHSL CTLEFEAHRP 250 260 270 280 290 300 LYDWVLDNIP ALHATRPRQY EFSRLELLYT ITSKRKLNQL VVEKHVSGWD DPRMPTISGM 310 320 330 340 350 360 RRRGYTPEGV RLFAKRAGIS KSENIVDMSV LEGAIREELE NSAPRLMAVL NPLKVTLTNF 370 380 390 400 410 420 QAGKTQSRRA AFHPNHEEMG DREVPVSQTI YIEADDFAEN PPKGFKRLIP GGEVRLRHGY 430 440 450 460 470 480 VIKCGEVVKD EAGNVVELKC SIDHDTLGKN PEGRKVKGVI HWVSAEHAAE IKVRLYDRLF 490 500 510 520 530 540 TVERPGAVRG EDGEYLPFTD FLNPESVKEI TAYAEPAAKD LPAESRWQFE RIGYFVTDRQ 550 560 DHGKDTPVFN RTVTLKDSWQ PK

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700825 / FA 1090.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004969 Genomic DNA. Translation: AAW89877.1.
RefSeq	YP_208289.1. NC_002946.2.
3D structure databases
ProteinModelPortal	Q5F7G0.
SMR	Q5F7G0. Positions 11-558.
ModBase	Search...
Protein-protein interaction databases
STRING	242231.NGO1218.
Proteomic databases
PRIDE	Q5F7G0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW89877; AAW89877; NGO1218.
GeneID	3281964.
KEGG	ngo:NGO1218.
PATRIC	20335747. VBINeiGon24812_1432.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	NGON242231:GI2G-1129-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_NEIG1

Accession

Primary (citable) accession number: Q5F7G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 29, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program