Reviewed, UniProtKB/Swiss-Prot Q5F5C8 (BICOA_NEIG1)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme birA/coaX
Including the following 2 domains:
    1- Recommended name:
            Biotin--[acetyl-CoA-carboxylase] synthetase
              EC=6.3.4.15
        Alternative name(s):
            Biotin--protein ligase
    2- Recommended name:
            Type III pantothenate kinase
              EC=2.7.1.33
        Alternative name(s):
            Pantothenic acid kinase
            PanK-III

Gene names

Name:	birA/coaX
Ordered Locus Names:	NGO2001

Organism

Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) [Complete proteome] [HAMAP]

Taxonomic identifier

242231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins By similarity. Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis By similarity.
Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]. HAMAP MF_01274 ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate. HAMAP MF_01274
Cofactor	Monovalent cations By similarity.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5. HAMAP MF_01274
Subcellular location	Cytoplasm Potential.
Sequence similarities	In the N-terminal section; belongs to the biotin--protein ligase family. In the C-terminal section; belongs to the type III pantothenate kinase family.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Kinase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: HAMAP positive regulation of transcription Inferred from electronic annotation. Source: InterPro protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC pantothenate kinase activity Inferred from electronic annotation. Source: HAMAP transcription activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Bifunctional enzyme birA/coaX HAMAP MF_01274		PRO_0000270882
Regions
	Nucleotide binding	344 – 351	8	ATP By similarity
	Region	1 – 329	329	Biotin--protein ligase HAMAP MF_01274
	Region	336 – 592	257	Type III pantothenate kinase HAMAP MF_01274
	Region	433 – 436	4	Substrate binding By similarity
Sites
	Active site	435	1	Proton acceptor Potential
	Binding site	426	1	Substrate By similarity
	Binding site	458	1	ATP Potential
	Binding site	508	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F5C8-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1FEA35BF638D2BF6	FASTA	592	64,167
	10 20 30 40 50 60 MTVLKPSHWR VLAELADGLP QHVSQLAREA DMKPQQLNGF WQQMPAHIRG LLRQHDGYWR 70 80 90 100 110 120 LVRPLAVFDA EGLRDLGERS GFQTALKHEC ASSNDEILEL ARIAPDKAHK TICVTHLQSK 130 140 150 160 170 180 GRGRQGRKWS HRLGECLMFS FGWAFDRPQY ELGSLSPVAA LACRRALGCL GLETQIKWPN 190 200 210 220 230 240 DLVVGRDKLG GILIETVRAG GKTVAVVGIG INFVLPKEVE NAASVQSLFQ TASRRGNADA 250 260 270 280 290 300 AVLLETLLAE LGAVLEQYAE EGFAPFLNEY ETANRDHGKA VLLLRDGETV CEGTVKGVDG 310 320 330 340 350 360 RGVLHLETAE GEQTVVSGEI SLRPDNRSVS VPKRPDSERF LLLEGGNSRL KWAWVENGTF 370 380 390 400 410 420 ATVGSAPYRD LSPLGAEWAE KADGNVRIVG CAVCGESKKA QVKEQLARKI EWLPSSAQAL 430 440 450 460 470 480 GIRNHYRHPE EHGSDRWFNA LGSRRFSRNA CVVVSCGTAV TVDALTDDGH YLGGTIMPGF 490 500 510 520 530 540 HLMKESLAVR TANLNRPAGK RYPFPTTTGN AVASGMMDAV CGSIMMMHGR LKEKNGAGKP 550 560 570 580 590 VDVIITGGGA AKVAEALPPA FLAENTVRVA DNLVIHGLLN LIAAEGGESE HA

References

[1]

"The complete genome sequence of Neisseria gonorrhoeae." Lewis L.A., Gillaspy A.F., McLaughlin R.E., Gipson M., Ducey T.F., Ownbey T., Hartman K., Nydick C., Carson M.B., Vaughn J., Thomson C., Song L., Lin S., Yuan X., Najar F., Zhan M., Ren Q., Zhu H. , Qi S., Kenton S.M., Lai H., White J.D., Clifton S., Roe B.A., Dyer D.W. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004969 Genomic DNA. Translation: AAW90609.1.
RefSeq	YP_209021.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F5C8.
Genome annotation databases
GeneID	3282623.
GenomeReviews	Gene locus NGO2001 in contig AE004969_GR.
KEGG	ngo:NGO2001.
NMPDR	fig|242231.4.peg.1872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5F5C8.
OMA	HECASSN.
Enzyme and pathway databases
BioCyc	NGON242231:NGO2001-MON.
Family and domain databases
HAMAP	MF_01274. Fused. [Tree]
InterPro	IPR004619. Baf. IPR004408. Biotin_CoA_COase_ligase. IPR003142. BPL_C. IPR004143. BPL_LipA_LipB. [Graphical view]
PANTHER	PTHR12835. BirA_ligase. 1 hit.
Pfam	PF02237. BPL_C. 1 hit. PF03099. BPL_LipA_LipB. 1 hit. PF03309. Bvg_acc_factor. 1 hit. [Graphical view]
TIGRFAMs	TIGR00671. baf. 1 hit. TIGR00121. birA_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BICOA_NEIG1

Accession

Primary (citable) accession number: Q5F5C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)