Reviewed, UniProtKB/Swiss-Prot Q5F408 (SYCC_CHICK)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	CARS
ORF Names:	RCJMB04_3o5

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Cysteinyl-tRNA synthetase, cytoplasmic		PRO_0000250745
Regions
	Motif	57 – 67	11	"HIGH" region
	Motif	406 – 410	5	"KMSKS" region
Sites
	Metal binding	55	1	Zinc By similarity
	Metal binding	348	1	Zinc By similarity
	Metal binding	373	1	Zinc By similarity
	Metal binding	377	1	Zinc By similarity
	Binding site	409	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F408-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6FAFFC0426BDC07C	FASTA	748	85,801
	10 20 30 40 50 60 MAAAPAEQGK GKRVQPPWSP PEGTKHSRLC LYNSLTRNKE IFQPQNGKKV TWYCCGPTVY 70 80 90 100 110 120 DASHMGHARS YISFDILRRI LRDYFKFDVF YCMNITDIDD KIIKRTRQNY LFEQYRKNKS 130 140 150 160 170 180 TPAQLLEDVK TASELFSVKL SETTDPDKRQ MLEKIQNAVK SAFDPLQEAV QAKLPAEEIS 190 200 210 220 230 240 RCHEILLEEA KDLLSDWLDS KFGSQVTDNS IFSELPKFWE GEFHKDMEAL NVLPPDVLTR 250 260 270 280 290 300 VSEYVPEIVA FVKKIVDNGY GYVSNGSVYF DTVKFGSSEK HSYAKLVPEA VGDQKALQEG 310 320 330 340 350 360 EGDLSISADR LCEKHSPNDF ALWKSSKPGE PSWDSPWGKG RPGWHIECSA MAGSILGESM 370 380 390 400 410 420 DIHGGGFDLR FPHHDNELAQ SEAYFENDNW VRYFLHTGHL TIAGCKMSKS LKNFITIKDA 430 440 450 460 470 480 LQKHTARQLR LAFLMHSWKD TLDYSSNTME SAIQYEKFMN EFFLNVKDIL RAPTDVTGQF 490 500 510 520 530 540 QKWENQEAEL NKNFYDKKAA IHEALCDNVD TRSVLEEMRS LVSQSNSYIA AKKSARQMPN 550 560 570 580 590 600 RLLLENISCY LTQMLKIFGA IESDDAIGFP VGGNNQNINI ESTVMPYLQV LSEFREGVRQ 610 620 630 640 650 660 IAREKKVTEV LQLSDALRDD ILPELGVRFE DHEGLPTVVK LVDKDTLLKE REEKKKIEEE 670 680 690 700 710 720 KKRKKEEAAR KKQEQEAAKL AKMKIPPHEM FKSEHDKYSK FDENGFPTHD TEGKELSKGQ 730 740 IKKLKKLYET QEKLYKEYLQ MVQNGSAN

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ851492 mRNA. Translation: CAH65126.1.
IPI	IPI00585125.
RefSeq	NP_001012601.1.
UniGene	Gga.22936
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F408.
Genome annotation databases
Ensembl	ENSGALT00000010375; ENSGALP00000010361; ENSGALG00000006427; Gallus gallus. [Genome view]
GeneID	423086.
KEGG	gga:423086.
Organism-specific databases
CTD	423086.
Phylogenomic databases
HOVERGEN	Q5F408.
Enzyme and pathway databases
BRENDA	6.1.1.16. 4.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_CHICK

Accession

Primary (citable) accession number: Q5F408

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 15, 2005
Last modified:	September 1, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)