Q5F407 (MGT4A_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.145

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name=GlcNAc-T IVa
Short name=GnT-IVa
Short name=N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa

Gene names

Name:	MGAT4A
ORF Names:	RCJMB04_3o8

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A		PRO_0000288591
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 535	508	Lumenal Potential
	Coiled coil	28 – 54	27	Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5F407 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 2ADFEA9847BDE6F8	FASTA	535	61,566
	10 20 30 40 50 60 MRLRNGTVAT VLVFITTFLS LSWYTAWQNG KEKLIAYQRE FHALKERLRI AEHRTLQRSS 70 80 90 100 110 120 ELNAILEQFR RAVAETNGSK NALNNFSDET LKLLKELTSK KSLQVPNIYY HLPHLLKNEG 130 140 150 160 170 180 SLQPSVQVGL GRTGVSIVMG IPTVKRKVKS YLTETLHSLI DKLSPEEKLD CVMVVFIGET 190 200 210 220 230 240 DLDYVNNVVA SLEKEFSTEI NSGLVEVIAP PATYYPDLTN LKETFGDSKE RVRWRTKQNL 250 260 270 280 290 300 DYCFLMMYAQ KKGVYYIQLE DDIVVKQNYF STIKNFALQL ASEDWMILEF SQLGFIGKMF 310 320 330 340 350 360 QSPDITLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKSNL RIRFRPSLFQ 370 380 390 400 410 420 HVGLHSSLAG KIQKLTDKDF LKPLLHKIHV NPPAEVSTSL KVYQGHTLEK TYVGEDFFWA 430 440 450 460 470 480 VTPVAGDYIL FKFDKPVNVE RYLFHSGNPE HPGDILLNTT VEVLPFQNEE LVLSKETKDK 490 500 510 520 530 RLEDGYFRIG KFENGVAEGT VDPSLNPISS FRLSVIQNSA VWAILNEIHI KKMTN

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ851493 mRNA. Translation: CAH65127.1.
IPI	IPI00601503.
RefSeq	NP_001012842.1. NM_001012824.1.
UniGene	Gga.29144.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F407.
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000027052; ENSGALP00000027001; ENSGALG00000016754.
GeneID	418692.
KEGG	gga:418692.
Organism-specific databases
CTD	11320.
Phylogenomic databases
eggNOG	veNOG10896.
GeneTree	ENSGT00550000074578.
HOGENOM	HBG506643.
HOVERGEN	HBG053194.
InParanoid	Q5F407.
OMA	SSEDWMI.
OrthoDB	EOG428219.
PhylomeDB	Q5F407.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
KO	K00738.
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4A_CHICK

Accession

Primary (citable) accession number: Q5F407

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 15, 2005
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program