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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Gene namesi
Name:MGAT4A
ORF Names:RCJMB04_3o8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 535508LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5F407.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000027001.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili28 – 5427Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ5F407.
KOiK00738.
OMAiLQVPNIY.
OrthoDBiEOG70ZZN3.
PhylomeDBiQ5F407.
TreeFamiTF324570.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5F407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT VLVFITTFLS LSWYTAWQNG KEKLIAYQRE FHALKERLRI
60 70 80 90 100
AEHRTLQRSS ELNAILEQFR RAVAETNGSK NALNNFSDET LKLLKELTSK
110 120 130 140 150
KSLQVPNIYY HLPHLLKNEG SLQPSVQVGL GRTGVSIVMG IPTVKRKVKS
160 170 180 190 200
YLTETLHSLI DKLSPEEKLD CVMVVFIGET DLDYVNNVVA SLEKEFSTEI
210 220 230 240 250
NSGLVEVIAP PATYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
260 270 280 290 300
KKGVYYIQLE DDIVVKQNYF STIKNFALQL ASEDWMILEF SQLGFIGKMF
310 320 330 340 350
QSPDITLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKSNL
360 370 380 390 400
RIRFRPSLFQ HVGLHSSLAG KIQKLTDKDF LKPLLHKIHV NPPAEVSTSL
410 420 430 440 450
KVYQGHTLEK TYVGEDFFWA VTPVAGDYIL FKFDKPVNVE RYLFHSGNPE
460 470 480 490 500
HPGDILLNTT VEVLPFQNEE LVLSKETKDK RLEDGYFRIG KFENGVAEGT
510 520 530
VDPSLNPISS FRLSVIQNSA VWAILNEIHI KKMTN
Length:535
Mass (Da):61,566
Last modified:March 15, 2005 - v1
Checksum:i2ADFEA9847BDE6F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ851493 mRNA. Translation: CAH65127.1.
RefSeqiNP_001012842.1. NM_001012824.1.
XP_015133201.1. XM_015277715.1.
UniGeneiGga.29144.

Genome annotation databases

EnsembliENSGALT00000027052; ENSGALP00000027001; ENSGALG00000016754.
GeneIDi418692.
KEGGigga:418692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ851493 mRNA. Translation: CAH65127.1.
RefSeqiNP_001012842.1. NM_001012824.1.
XP_015133201.1. XM_015277715.1.
UniGeneiGga.29144.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000027001.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Proteomic databases

PaxDbiQ5F407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000027052; ENSGALP00000027001; ENSGALG00000016754.
GeneIDi418692.
KEGGigga:418692.

Organism-specific databases

CTDi11320.

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ5F407.
KOiK00738.
OMAiLQVPNIY.
OrthoDBiEOG70ZZN3.
PhylomeDBiQ5F407.
TreeFamiTF324570.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-GGA-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiQ5F407.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiMGT4A_CHICK
AccessioniPrimary (citable) accession number: Q5F407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 15, 2005
Last modified: June 8, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.