Reviewed, UniProtKB/Swiss-Prot Q5F3X0 (LCLT1_CHICK)

Names and origin

Protein names

Recommended name:
    Lysocardiolipin acyltransferase 1
    EC=2.3.1.51
    EC=2.3.1.-

Gene names

Name:	LCLAT1
Synonyms:	LYCAT
ORF Names:	RCJMB04_5b22

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Required for establishment of the hematopoietic and endothelial lineages By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Developmental protein Transferase
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Lysocardiolipin acyltransferase 1		PRO_0000291579
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	46 – 66	21	Potential
	Transmembrane	302 – 322	21	Potential
	Transmembrane	336 – 358	23	Potential
	Motif	85 – 90	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q5F3X0-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 655199AD448BF7D0	FASTA	378	44,448
	10 20 30 40 50 60 MVSWKGIYFV VALFLGSFFG SIFMLGPFLP LMFISPAWYR WITDRIVATW LTLPVALLEM 70 80 90 100 110 120 VFGAKVVVTG DGFIPGERSV IIMNHRTRMD WMFLWNCLLR YSYLRLEKIC LKSSLKSIPG 130 140 150 160 170 180 FGWAMQVAAF IFIQRKWEDD KSHFENMLHY FCDIHEPLQL LIFPEGTDLT ANTKARSNDF 190 200 210 220 230 240 AEKNGLRKYE YVLHPRTTGF TFVVECLREG NNLDAIHDIT VAYPQNIPQT EKHLLNGNFP 250 260 270 280 290 300 KEIHFHVQRY PIETVPTSKE ELQLWCQKRW EEKEERLRRF YEGGKCFDET GQSIIPPCKS 310 320 330 340 350 360 ELRVLAVKCI SLLYWTVFPM GTFALLYLYS FARWYFAAMI IIFVAQQKIF GGLELIELAC 370 HQYFKKQQKH DDTKMKKK

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ851530 mRNA. Translation: CAH65164.1.
IPI	IPI00584990.
RefSeq	NP_001026210.1.
UniGene	Gga.7898
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5F3X0.
Genome annotation databases
Ensembl	ENSGALT00000014742; ENSGALP00000014726; ENSGALG00000009056; Gallus gallus. [Genome view]
GeneID	421303.
KEGG	gga:421303.
Organism-specific databases
CTD	421303.
Phylogenomic databases
HOGENOM	Q5F3X0.
HOVERGEN	Q5F3X0.
OMA	CDIREPL.
Enzyme and pathway databases
BRENDA	2.3.1.51. 4.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCLT1_CHICK

Accession

Primary (citable) accession number: Q5F3X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)