Q5F3N1 (PIMT_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase
Short name=PIMT
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase

Gene names

Name:	PCMT1
ORF Names:	RCJMB04_11o11

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 228	227	Protein-L-isoaspartate(D-aspartate) O-methyltransferase		PRO_0000253635
Sites
	Active site	60	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F3N1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5F65A50FA1739916	FASTA	228	24,691
	10 20 30 40 50 60 MAWKSGGASH SELIHNLRKN GIIKSDKVFE VMLATDRCHY AKYNPYMDSP QSIGFQATIS 70 80 90 100 110 120 APHMHAYALE LLSDQLHEGA KALDVGSGSG ILTACFSRMV GPKGQVVGID HIKELVDDSI 130 140 150 160 170 180 NNVKKDDPTL LSSGRVKLIV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI 190 200 210 220 LPVGPAGGNQ MLEQYDKLED GSVKMKPLMG VIYVPLTDKE KQWSRDEL

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ851619 mRNA. Translation: CAH65253.1.
IPI	IPI00574350.
RefSeq	NP_001026696.1. NM_001031525.1.
UniGene	Gga.16623.
3D structure databases
HSSP	HSSP built from PDB template 1I1N based on UniProtKB P22061.
ProteinModelPortal	Q5F3N1.
SMR	Q5F3N1. Positions 3-225.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000036798.
Proteomic databases
PaxDb	Q5F3N1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	428607.
KEGG	gga:428607.
Organism-specific databases
CTD	5110.
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
HOVERGEN	HBG004483.
InParanoid	Q5F3N1.
KO	K00573.
Gene expression databases
ArrayExpress	Q5F3N1.
Family and domain databases
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20829551.

Entry information

Entry name

PIMT_CHICK

Accession

Primary (citable) accession number: Q5F3N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 50 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program