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Protein

Ribosomal protein S6 kinase alpha-5

Gene

RPS6KA5

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and that contributes to gene activation by histone phosphorylation. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation at Ser-350, Thr-571 and Thr-690 by MAP kinases, and by further autophosphorylation of Ser-202, Ser-366 and Ser-371 by the activated C-terminal kinase domain. The active N-terminal kinase domain finally phosphorylates downstream substrates, as well as Ser-740, Ser-742 and Ser-748 in its own C-terminal region (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711ATPPROSITE-ProRule annotation
Active sitei167 – 1671Proton acceptorBy similarity
Binding sitei445 – 4451ATPPROSITE-ProRule annotation
Active sitei534 – 5341Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 539ATPPROSITE-ProRule annotation
Nucleotide bindingi422 – 4309ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S6 kinase alpha-5 (EC:2.7.11.1)
Short name:
S6K-alpha-5
Gene namesi
Name:RPS6KA5
ORF Names:RCJMB04_14g1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 789789Ribosomal protein S6 kinase alpha-5PRO_0000232734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei202 – 2021Phosphoserine; by autocatalysisBy similarity
Modified residuei350 – 3501PhosphoserineBy similarity
Modified residuei366 – 3661Phosphoserine; by autocatalysisBy similarity
Modified residuei371 – 3711Phosphoserine; by autocatalysisBy similarity
Modified residuei571 – 5711PhosphothreonineBy similarity
Modified residuei690 – 6901PhosphothreonineBy similarity
Modified residuei740 – 7401Phosphoserine; by autocatalysisBy similarity
Modified residuei742 – 7421Phosphoserine; by autocatalysisBy similarity
Modified residuei748 – 7481Phosphoserine; by autocatalysisBy similarity

Post-translational modificationi

Ser-366 and Thr-571 phosphorylation is required for kinase activity. Ser-366 and Ser-202 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-350, Thr-571 and Thr-690 by MAP kinases. Autophosphorylated at Ser-740, Ser-742 and Ser-748 by the N-terminal kinase domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5F3L1.

Expressioni

Tissue specificityi

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000017378.

Structurei

3D structure databases

ProteinModelPortaliQ5F3L1.
SMRiQ5F3L1. Positions 15-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 308270Protein kinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini309 – 37769AGC-kinase C-terminalAdd
BLAST
Domaini416 – 677262Protein kinase 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Enzyme activity requires the presence of both kinase domains.By similarity

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 protein kinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ5F3L1.
KOiK04445.
OrthoDBiEOG76HQ0Z.
PhylomeDBiQ5F3L1.
TreeFamiTF313438.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016239. Ribosomal_S6_kinase_II.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000606. Ribsml_S6_kin_2. 1 hit.
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 2 hits.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00108. PROTEIN_KINASE_ST. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5F3L1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPSGSAQG SEWPLLTVTH ELRNANLTGH AEKVGIENFE LLKVLGTGAY
60 70 80 90 100
GKVFLVRKVS GHDAGKLYAM KVLKKATIVQ KAKTTEHTRT ERQVLEHIRQ
110 120 130 140 150
SPFLVTLHYA FQTDTKLHLI LDYINGGELF THLSQRERFS ENEVQIYIGE
160 170 180 190 200
IVLALEHLHK LGIIYRDIKL ENILLDSDGH VVLTDFGLSK EFLTDENERA
210 220 230 240 250
YSFCGTIEYM APDIVRGGDT GHDKAVDWWS VGVLMYELLT GASPFTVDGE
260 270 280 290 300
KNSQAEISRR ILKSEPPYPQ EMSALSKDII QRLLMKDPKK RLGCGPTDAD
310 320 330 340 350
EIKQHPFFQN INWDDLAAKK VPAPFKPVIR DELDVSNFAE EFTEMDPTYS
360 370 380 390 400
PAATPQTSEK IFQGYSFVAP SILFKRNAAT VDPVQFYVGD ERPGTTTIAR
410 420 430 440 450
SAMMKDSPFF QHYELDLKEK PLGEGSFSIC RKCLHKKTSQ EYAVKIISKR
460 470 480 490 500
MEANTQREIT ALKLCEGHPN VVKLHEVYHD QLHTFLVMEL LKGGELLERI
510 520 530 540 550
QKKQHFSETE ASHIMRRLVS AVSHMHDVGV VHRDLKPENL LFTDETDNSE
560 570 580 590 600
IKIIDFGFAR LKPPDNQPLK TPCFTLHYAA PELFNHNGYD ESCDLWSLGV
610 620 630 640 650
ILYTMLSGQV PFQSQDKSLT CTSALEIMKK IKKGEFSFEG EAWKNVSEEA
660 670 680 690 700
KELIQGLLTV DPNKRIKMSS LRYNEWLQDG SQLSSNPLMT PDNLGSSGAA
710 720 730 740 750
VHTYVKATFH AFNKYKREGF CLQNVDKAPL AKRRKMKKTS TSTETRSSSS
760 770 780
ESSHSSSSHS HGKTTPTKTL QPTNPTDSNN PETIFQFSD
Length:789
Mass (Da):89,040
Last modified:March 15, 2005 - v1
Checksum:i2FA3FC00B6098B1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ851639 mRNA. Translation: CAH65273.1.
RefSeqiNP_001012605.1. NM_001012587.2.
UniGeneiGga.14856.

Genome annotation databases

GeneIDi423408.
KEGGigga:423408.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ851639 mRNA. Translation: CAH65273.1.
RefSeqiNP_001012605.1. NM_001012587.2.
UniGeneiGga.14856.

3D structure databases

ProteinModelPortaliQ5F3L1.
SMRiQ5F3L1. Positions 15-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000017378.

Proteomic databases

PaxDbiQ5F3L1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi423408.
KEGGigga:423408.

Organism-specific databases

CTDi9252.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ5F3L1.
KOiK04445.
OrthoDBiEOG76HQ0Z.
PhylomeDBiQ5F3L1.
TreeFamiTF313438.

Miscellaneous databases

NextBioi20825882.
PROiQ5F3L1.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016239. Ribosomal_S6_kinase_II.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000606. Ribsml_S6_kin_2. 1 hit.
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 2 hits.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00108. PROTEIN_KINASE_ST. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiKS6A5_CHICK
AccessioniPrimary (citable) accession number: Q5F3L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: March 15, 2005
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Enzyme activity requires the presence of both kinase domains.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.